Abstract: Technology with microsatellite was used to analyze the genetic difference between disease-resistant and disease-susceptible groups of GIFT strain. The results show that there were 20 loci successfully amplified with stable target bands within 30 tested loci to identify the polymorphism for the tested GIFT strain. The average alleles in disease-resistant was 2. 5 slightly higher than that of 2. 4 in disease-rusceptible group. Then the average of observed heterozygosity(Ho) and expected heterozygosity(He) were 0. 469 and 0. 441, 0. 440 and 0. 476 respectively and the polymorphism information content (PIC) was 0. 359 and 0. 379. Genetic similarity index and genetic distance was 0. 055 and 0. 054 respectively. The locus UNH845,UNH189 and GM180 showed significant departure from Hardy Weinberg equilibrium after sequential bonferroni correction(P<0. 05)in two groups. GM251 and GM462 sites differences of allele fragment(135,198 bp) were positively related to disease-resistance GIFT strain. Above mentioned results indicated that the genetic diversity between disease-resistant and disease}usceptible group of GIFT strain was not significantly different, and the GM251 and GM462 gene fragment with its differential bands from the PCR ampliftcation can be used as the molecular marker for the disease}esistant GIFT strain molecular assistant selection.

Key words: GIFT strain, Microsatellite, Genetic diversity, Genetic marker

CLC Number: 

• Q78