Comparative Analysis of Genetic Diversity of Medicago ruthenica (L.) in China Using AFLP and SSR Markers

doi:10.7668/hbnxb.2017.02.019

Abstract

Abstract: In order to select new breeding materials of Ruthenia Medic with outstanding.In this study,we used AFLP and SSR markers to investigate the genetic diversity in 15 wild Ruthenia Medic accessions from Seven provinces and autonomous regions of China.A total of 109 alleles were detected using 18 pairs of SSR primers,and 640 bands containing 472 polymorphic bands were revealed using 8 pairs of AFLP primers.The mean Nei's gene diversity,Shannon's information index and genetic differentiation coefficient obtained by AFLP were higher than those obtained by SSR.The genetic distances and similarities among the 15 wild accessions demonstrated their close phylogenetic relationship.Dendrograms with arithmetic average based on both AFLP and SSR data clustering showed that the 15 accessions could be grouped into four main clusters.However,the order of clustering did not completely agree with the geographical distribution.A similar clustering pattern was obtained using principal coordinate analysis.The Mantel test identified a highly significant correlation of the AFLP and SSR data.Thus,combined AFLP and SSR more efficiently estimated and validated the genetic diversity of the Ruthenia Medic accessions.These results can aid the formulation of breeding and germplasm conservation strategies for Ruthenia Medic.

Key words: M. ruthenica, Genetic diversity, AFLP, SSR

CLC Number:

S52.03
Q78

LI Hongyan, LU Xinxiong, XIN Xia, LI Zhiyong, LI Jun, HUANG Fan, LIU Lei. Comparative Analysis of Genetic Diversity of Medicago ruthenica (L.) in China Using AFLP and SSR Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(2): 124-130. doi: 10.7668/hbnxb.2017.02.019.

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