Abstract: To determine the main factor of promoting plant growth from Paecilomyces lilacinus PL-HN-16.Salkowski colorimetric test,wheat coleoptiles test and HPLC were used to detect IAA.Activities of the protein were test which was separed by ion exchang chromatography from the culture bath.Results of Salkowski colorimetric test,wheat coleoptiles test and HPLC test showed that strain PL-HN-16 did not produce IAA.The strain could increase the soluble phosphorus but it was not the main reason to promote plant growth.Protein test results showed that the protein which molecular weight was about 14 kDa could promote plant growth when the content below 0.10 μg/mL and dose-relative positive correlation,the percent of promoting was increased by 13.15%.The protein might inhabit plant growth when the content was higher than 0.10 μg/mL.The protein which MW was about 14 kDa produced by the strain PL-NH-16 was the factor of promoting plant growth.There was not any report about heterologous protein affected as phytohormone IAA to plant.

Key words: PL-HN-16, Plant growth regulators, Promoting plant growth, Purification

CLC Number: 

• S432.4