Isolation and Purification of Tobacco Wildfire Bacteria Using PCR Technique

doi:10.7668/hbnxb.2015.S1.059

Abstract

Abstract: Specific PCR identification primers were designed in this study to purify collected Pseudomonas syringae pv.Tabaci isolates using PCR technique.The pathogenicity of purified Pseudomonas syringae pv.Tabaci isolates were evaluated by inoculating tobacco plants.The obtained results verified the specificity of identification PCR primers and proved the practicability and reliability of the isolation and purification of Pseudomonas syringae pv.Tabaci using PCR technique.

Key words: Polymerase chain reaction (PCR), Tobacco wildfire disease, Separation, Purification

CLC Number:

S432.4

ZHANG Meng, WAN Xiu-qing, QIAO Chan, LI Ruo, LI Xiu-lan, SUN Jian-ping, GUO Zhao-kui. Isolation and Purification of Tobacco Wildfire Bacteria Using PCR Technique[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 329-332. doi: 10.7668/hbnxb.2015.S1.059.

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http://www.hbnxb.net/EN/Y2015/V30/IS1/329

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