Identification and Main Biological Characteristics of Sclerotinia Rot Pathogen in Chinese Cabbage

doi:10.7668/hbnxb.2015.S1.058

Abstract

Abstract: In order to clarify the pathogen type of Chinese cabbage Sclerotinia rot and further study its main biological characteristics,A strain XN001 of Chinese cabbage Sclerotinia rot was isolated from Chinese cabbage plant infected by pathogen and identified its type through pathogenicity test,morphology observation and analysis of nucleotide sequence of internal transcribed spacer (ITS).In addition,the main biological characteristics of pathogen were also studied by changing culturing conditions of mycelial and sclerotia.The results showed that:The pathogen of Chinese cabbage Sclerotinia rot was as same as Sclerotinia rot of colza and both of them were Sclerotinia sclerotiorum.It was suitable for strain XN001 growing when the pH value was between 4 to 11,the favourable pH value for mycelium growth was 4;The optimal temperature for its mycelium growth was between 20 ℃ and 25 ℃;The optimal temperature for inducing sclerotia germination was between 4 ℃ and 10 ℃,and the sclerotia germination was best when treated for between 3 and 5 weeks under 4 ℃;It was suitable for sclerotia germination when the culturing temperature was between 15 ℃ and 25 ℃ after low temperature treated.Obviously,the pathogen of Chinese cabbage Sclerotinia rot was S.sclerotiorum,it was suitable for growth at the conditions of acid and ambient temperature and the germination of sclerotia need low temperature induction.

Key words: Chinese cabbage sclerotinia rot, Sclerotinia sclerotiorum, Identification, ITS, Biological characteristics

CLC Number:

S432.4

SUN Jing-xian, ZHANG Lu-gang. Identification and Main Biological Characteristics of Sclerotinia Rot Pathogen in Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 321-328. doi: 10.7668/hbnxb.2015.S1.058.

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