A Simple and High Quality of Total RNA Extraction Method for Pholiota adiposa

doi:10.7668/hbnxb.2016.S1.013

Abstract

Abstract: Analyzing the function of RNA via RNA-seq technique is a hotspot research currently.In order to study the medicinal value of Pholiota adiposa,such as antitumor mechanism research,on the level of the transcriptome,one need extraction and separation of high purity,high quality Pholiota adiposa RNA samples. This research utilize TRIzol one-step method with liquid nitrogen grinding to extract Pholiota adiposa total RNA.Subsequently,one compared differences of the product between with our method and without. Following,the results are evaluated and processed statistical analysis. Results showed the extracted RNA concentration of the samples via our method was far higher than that without and display a high purity(OD_260/280=2). At the same time,agarose electrophoresis experiment exhibited that all fluorescence intensity of three samples was approximately equal to 2,respectively 1.8,1.9,1.9,and all RIN value of three samples via Agilent 2100 are further greater than the standard of 6.8,respectively 9.1,8.7,9.3,indicating that the extracted RNA products have perfect integrity. As a result,TRIzol one-step method with nitrogen grinding is a simple,efficient way and very suitable for extracting the total RNA of Pholiota adiposa.

Key words: RNA separation, Pholiota adiposa, RNA-seq, Antineoplastic

CLC Number:

Q78
S646.03

GUO Chengang, LI Shunguo, WEI Zhimin, XIA Xueyan. A Simple and High Quality of Total RNA Extraction Method for Pholiota adiposa[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 75-79. doi: 10.7668/hbnxb.2016.S1.013.

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http://www.hbnxb.net/EN/Y2016/V31/IS1/75

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