Transcriptome and Metabolome Analysis to Reveal the Mechanisms Responding to High Temperature Stress in Anthesis Stage of Maize

doi:10.7668/hbnxb.20190649

Abstract

Abstract: The aim of the study was to explore differential expression of genes and metabolites in Zhengdan 958 and Xianyu 335 exposed to high temperature for 7,14 d during grain filling stage and identify the key candidate genes,transcription factors and metabolites,so as to understand molecular mechanism of high temperature tolerance in maize. The ear-leaf samples collected before high temperature treated(CK),7 d high temperature treated,14 d from Zhengdan 958 and Xianyu 335 were used for RNA-sequencing using Illumina HiSeq^TM 2500 high-throughput sequencing technology. Metabolome variations analyzed using liquid-chromatography-mass spectrometry-based metabolomics. A total of 214.81 Gb clean sequence was obtained by transcriptome sequencing. In Zhengdan 958,49 DEGs were detected after 7 days of high temperature treated,of which 24 were up-regulated genes. 306 DEGs were detected after 14 d,130 of which were up-regulated genes. 1 462 DEGs were detected from 7 d samples compared with 14 d samples,of which 647 were up-regulated genes. In Xianyu 335,381 DEGs were detected after 7 days of high temperature treated,of which 164 were up-regulated genes. 299 DEGs were detected after 14 d,226 of which were up-regulated genes. 2 481 DEGs were detected from 7 d treated samples compared with 14 d treated samples,of which 1 275 were up-regulated genes. Comparing Zhengdan 958 samples treated for 7 d with Xianyu 335 samples treated for 7 d,6 646 DEGs were detected,of which 3 253 up-regulated genes. Comparing Zhengdan 958 samples treated for 14 d with Xianyu 335 samples treated for 14 d,5 958 DEGs were detected,of which 3 110 up-regulated genes. A total of 654 metabolites were detected by metabolomics sequencing. In Zhengdan 958,28 DEMs were detected after 7 days of high temperature treated and were annotated into 5 metabolic pathways. 54 DEMs were detected after 14 days,9 of which were up-regulated,and were annotated into 13 metabolic pathways. In Xianyu 335, 98 DEMs were detected after 7 d of high temperature treated,43 of which were up-regulated and were annotated into 24 metabolic pathways. 38 DEMs were detected after 14 d,14 of which were up-regulated,and were annotated into 13 metabolic pathways. Compared Zhengdan 958 with Xianyu 335,144 DEMs were detected after 7 days,81 of which were up-regulated,and were annotated into 36 metabolic pathways.158 DEMs were detected after 14 days,81 of which were up-regulated,and were annotated into 40 metabolic pathways.

Key words: Maize, High temperature stress, Anthesis stage, Illumina RNA-sequencing, Metabolomics profiling, Differentially expressed gene(DEG)

CLC Number:

S513

LI Chuan, QIAO Jiangfang, HUANG Lu, ZHANG Meiwei, ZHANG Panpan, NIU Jun, LIU Jingbao. Transcriptome and Metabolome Analysis to Reveal the Mechanisms Responding to High Temperature Stress in Anthesis Stage of Maize[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(1): 8-21. doi: 10.7668/hbnxb.20190649.

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References

[1] Leng G Y,Tang Q H,Rayburg S. Climate change impacts on meteorological,agricultural and hydrological droughts in China[J]. Global and Planetary Change,2015,126(3):23-34.doi:10.1016/j.gloplacha.2015.01.003.
[2] 权畅,景元书,谭凯炎. 高温热害对华北地区冬小麦灌浆和产量的影响[J]. 河南农业科学,2014,43(1):28-32.doi:10.15933/j.cnki.1004-3268.2014.01.016.
Quan C,Jing Y S,Tan K Y. Effects of heat damage on grain filling and yield of winter wheat in North China[J]. Journal of Henan Agricultural Science,2014,43(1):28-32.
[3] 赵鸿,王润元,尚艳,王鹤龄,张凯,赵福年,齐月,陈斐.粮食作物对高温干旱胁迫的响应及其阈值研究进展与展望[J]. 干旱气象,2016, 34(1):1-12.doi:10. 11755/j.issn.1006-7639(2016)-0l-0001.
Zhao H,Wang R Y,Shang Y,Wang H L,Zhang K,Zhao F N,Qi Y,Chen F. Progress and perspectives in studies on responses and thresholds of major food crops to high temperature and drought stress[J]. Journal of Arid meteorology,2016,34(1):1-12.
[4] Zandalinas S I,Mittler R,Balfagón D,Arbona V,Gómez-Candenas A. Plant adaptations to the combination of drought and high temperatures[J]. Physiologia Plantarum,2017,162(1):2-12.doi:10.1111/ppl.12540.
[5] Jha U C,Bora A,Singh N P. Heat stress in crop plants:its nature,impacts and integrated breeding strategies to heat tolerance[J]. Plant Breeding,2014,133(6):679-701.doi:10.1111/pbr.12217.
[6] 王占彪,王猛,尹小刚,张海林,褚庆全,文新亚,陈阜. 气候变化背景下华北平原夏玉米各生育期水热时空变化特征[J]. 中国生态农业学报,2015,23(4):473-481.doi:10.13930/j.cnki.cjea.141277.
Wang Z B,Wang M,Yin X G,Zhang H L,Chu Q Q,Wen X Y,Chen F. Spatiotemporal characteristics of heat and rainfall changes in summer maize season under climate change in the North China Plain[J]. Chinese Journal of Eco-Agriculture,2015,23(4):473-481.
[7] 郑云普,徐明,王建书,邱帅,王贺新. 玉米叶片气孔特征及气体交换过程对气候变暖的响应[J]. 作物学报,2015,41(4):601-612.doi:10.3724/SP.J.1006.2015.00601.
Zheng Y P,Xu M,Wang J S,Qiu S,Wang H X. Responses of the stomatal traits and gas exchange of maize leaves to climate warming[J]. Acta Agronomica Sinica,2015,41(4):601-612.
[8] 张吉旺,董树亭,王空军,刘鹏,胡昌浩.大田增温对夏玉米光合特性的影响[J]. 应用生态学报,2008,19(1):81-86.doi:10.3724/SP.J.1006.2015.00329.
Zhang J W,Dong S T,Wang K J,Liu P,Hu C H. Effects of increasing field temperature on photosynthetic characteristics of summer maize[J]. Chinese Journal of Applied Ecology,2008,19(1):81-86.
[9] Lobell D B,Hammer G L,Mclean G,Messina C,Roberts M J,Schlenker W. The critical role of extreme heat for maize production in the United States[J]. Nature Climate Change,2013,3(5):497-501.doi:10.1038/nclimate1832.
[10] 王亚梁,张玉屏,朱德峰,向镜,武辉,陈惠哲,张义凯. 水稻穗分化期高温胁迫对颖花退化及籽粒充实的影响[J]. 作物学报,2016,42(9):1402-1410.doi:10.3724/SP.J.1006.2016.01402.
W ang Y L,Zhang Y P,Zhu D F,Xiang J,Wu H,Chen H Z,Zhang Y K. Effect of heat stress on spikelet degeneration and grain filling at panicle initiation period of rice[J]. Acta Agronomica Sinica,2016,42(9):1402-1410.
[11] 谭凯炎,杨晓光,任三学,房世波.高温胁迫对华北地区冬小麦灌浆及产量的影响[J]. 生态学报,2015,35(19):6355-6361.doi:10.5846/stxb201402280346.
Tan K Y,Yang X G,Ren S X,Fang S B. Impact of high temperature stress at the grain-filling stage on winter wheat yield[J]. Acta Ecologica Sinica,2015,35(19):6355-6361.
[12] 周梦子,王会军,霍治国. 极端高温天气对玉米产量的影响及其与大气环流和海温的关系[J].气候与环境研究,2017,22(2):134-148.doi:10.3878/j.issn.1006-9585.2016.16119.
Zhou M Z,Wang H J,Huo Z G. The influence of heat stress on maize yield and its association with atmospheric general circulation and sea surface temperature[J]. Climatic and Environmental Research,2017,22(2):134-148.
[13] 李德,孙义,孙有丰. 淮北平原夏玉米花期高温热害综合气候指标研究[J]. 中国生态农业学报,2015,23(8):1035-1044.doi:10.13930/j.cnki.cjea.150061.
JP] Li D,Sun Y,Sun Y F. Use of integrated climatic index to determine high temperature damage to summer maize at florescence in the Huaibei Plain[J]. Chinese Journal of Eco-Agriculture,2015,23(8):1035-1044.
[14] 赵龙飞,李潮海,刘天学,王秀萍,僧姗姗. 花期前后高温对不同基因型玉米光合特性及产量和品质的影响[J]. 中国农业科学,2012,45(23):4947-4958.doi:10.3864/j.issn.0578-1752.2012.23.023.
Zhao L F,Li C H,Liu T X,Wang X P,Seng S S. Effect of high temperature during flowering on photosynthetic characteristics and grain yield and quality of different genotypes of maize(Zea may L.)[J]. Scientia Agricultura Sinica,2012,45(23):4947-4958.
[15] 赵霞,穆心愿,马智艳,刘天学,齐红志,丁勇,张凤启,张君,赵发欣,邢健伟,吴东洪,唐宝军. 不同玉米杂交种对花期高温、干旱复合胁迫的响应[J]. 河南农业科学,2017,46(8):32-37.doi:10.15933/j.cnki.1004-3268.2017.08.006.
Z hao X,Mu X Y,Ma Z Y,Liu T X,Qi H Z,Ding Y,Zhang F Q,Zhang J,Zhao F X,Xing J W,Wu D H,Tang B J. Response of different maize hybrids to high temperature and drought stresses at flowering stage[J]. Journal of Henan Agricultural Sciences,2017,46(8):32-37.
[16] 李树岩,王靖,余卫东,陈忠民. 气候变化对河南省夏玉米主栽品种发育期的影响模拟[J].中国农业气象,2015,36(4):479-488.doi:10.3969/j.issn.1000-6362.2015.04.012.
Li S Y,Wang J,Yu W D,Chen Z M. Modelling the impacts of climate change on phenology of representative maize varieties in Henan province[J]. Chinese Journal of Agrometeorology,2015,36(4):479-488.
[17] 阿里穆斯,于贵瑞. 植物光合作物模型参数的温度依存性研究进展[J]. 应用生态学报,2013,24(12):3588-3594.doi:10.13287/j.1001-9332.2013.0598.
Borjigidai A,Yu G R. Temperature dependence of parameters of plant photosynthesis models:A review[J].Chinese Journal of Applied Ecology,2013,24(12):3588-3594.
[18] 杨再强,李伶俐,殷剑敏,王学林,孙擎,顾礼力. 灌浆初期不同时长高温胁迫对早稻叶片光合和荧光参数的影响[J]. 中国农业气象,2014,35(1):80-84.doi:10.3969/j.issn.1000-6362.2014.01.012.
Yang Z Q,Li L L,Yin J M,Wang X L,Sun Q,Gu L L. Effects of different duration of high temperature stress during filling stages on leaf photosynthetic and fluorescence parameters of early rice[J]. Chenese Journal of Agrometeorology,2014,35(1):80-84.
[19] Lobell D B,Hammer G L,Mclean G,Messina C,Roberts M J,Schlenker W. The critical role of extreme heat for maize production in the United States[J]. Nature Climate Change,2013,3(5):497-501.doi:10.1038/nclimate1832.
[20] 张百富,张立军,樊金娟,崔震海,阮燕晔,朱延姝,汪澈,胡凯. 玉米幼苗不同叶位花环结构及叶绿素含量研究[J].河南农业科学,2011,40(2):33-35.doi:10.3969/j.issn.1004-3268.2011.02.009.
Zhang B F,Zhang L J,Fan J J,Cui Z H,Ruan Y Y,Zhu Y S,Wang C,Hu K. Comparative study on the kranz anatomy and chlorophyll content of different leaves in maize seedlings[J]. Journal of Henna Agricultural Sciences,2011,40(2):33-35.
[21] You J,Chan Z L. ROS regulation during abiotic stress responses in crop plants[J]. Frontiers in Plant Science,2015,6(1092):1-15.doi:10.3389/fpls.2015.01092.
[22] 邓茳明,熊格生,袁小玲,贾菲,刘志. 棉花不同耐高温品系的SOD、POD、CAT活性和MDA含量差异及其对盛花期高温胁迫的响应[J].棉花学报,2010,22(3):242-247.doi:10.3969/j.issn.1002-7807.2010.03.009.
Deng J M,Xiong G S,Yuan X L,Jia F,Liu Z. Differences in SOD,POD,CAT activities and MAD content and their responses to high temperature stress at peak flowering stage in cotton lines with different tolerance to high temperature[J]. Cotton Science,2010,22(3):242-247.
[23] 王永军,杨今胜,袁翠平,柳京国,李登海,董树亭. 超高产夏玉米花粒期不同部位叶片衰老与抗氧化酶特性[J].作物学报,2013,39(12):2183-2191.doi:10.3724/SP.J.1006.2013.02183.
Wang Y J,Yang J S,Yuan C P,Liu J G,Li D H,Dong S T. Characteristics of senescence and antioxidant enzyme activities in leaves at different plant parts of summer maize with the super-high yielding potential after anthesis[J]. Acta Agronomica Sinica,2013,39(12):2183-2191.
[24] 胡秀丽,李艳辉,杨海荣,刘全军,李潮海. HSP70可提高干旱高温复合胁迫诱导的玉米叶片抗氧化防护能力[J].作物学报,2010,36(4):636-644.doi:10.3724/SP.J.1006.2010.00636.
H u X L,Li Y H,Yang H R,Liu Q J,Li C H. Heat shock protein 70 may improve the ability of antioxidant defense induced by the combination of drought and heat in maize leaves[J]. Acta Agronomica Sinica,2010,36(4):636-644.
[25] 杨波,田露,李琦,宋晓卉,王兰兰. 水分和高温复合胁迫对水稻幼苗光合生理特性的影响[J]. 作物杂志,2015(5):111-115.doi:10.16035/j.issn.1001-7283.2015.05.021.
Yang B,Tian L,Li Q,Song X H,Wang L L. Effects of water stress and high temperature on photosynthetic physiological characteristics of rice seedlings[J]. Crops,2015(5):111-115.
[26] 李川,乔江方,朱卫红,代书桃,黄璐,张美微,刘京宝. 玉米自交系响应花粒期高温胁迫差异表达基因的分析[J]. 华北农学报,2019,34(1):1-11.doi:10.7668/hbnxb.201750986.
Li C,Qiao J F,Zhu W H,Dai S T,Huang L,Zhang M W,Liu J B. Differential expression of high temperature stress in anthesis stage related genes of maize inbred lines[J]. Acta Agriculturae Boreali-Sinica,2019,34(1):1-11.
[27] 王丰,程方民,刘奕,钟连进,张国平. 不同温度下灌浆期水稻籽粒内源激素含量的动态变化[J].作物学报,2006,32(1):25-29.doi:10.3321/j.issn:0496-3490.2006.01.005.
Wang F,Cheng F M,Liu Y,Zhong L J,Zhang G P. Dynamic changes of plant hormones in developing grains at rice filling stage under different temperatures[J]. Acta Agronomica Sinica,2006,32(1):25-29.
[28] 徐云姬,顾道健,张博博,张耗,王志琴,杨建昌. 玉米果穗不同部位籽粒激素含量及其与胚乳发育和籽粒灌浆的关系[J].作物学报,2013,39(8):1452-1461.doi:10.3724/SP.J.1006.2013.01452.
Xu Y J,Gu D J,Zhang B B,Zhang H,Wang Z Q,Yang J C. Hormone contents in kernels at different positions on an ear and their relationship with endosperm development and kernel filling in maize[J].Acta Agronomica Sinica,2013,39(8):1452-1461.
[29] 王继玥,余庭跃,张采波. 玉米转录组学研究进展[J]. 华北农学报,2014,29(S1):10-15.doi:10.7688/hbnxb.2014.
S1.003.
Wang J Y,Yu T Y,Zhang C B. Research progress on transcriptomics in maize[J]. Acta Agriculturae Boreali-Sinica,2014,29(S1):10-15.
[30] 张保仁,董树亭,胡昌浩,王空军. 高温对玉米籽粒淀粉合成及产量的影响[J]. 作物学报,2007,33(1):38-42.doi:10.3321/j.issn:0496-3490.2007.01.007.
Zhang B R,Dong S T,Hu C H,Wang K J. Effect of high air temperature during different growth stage on starch synthesis in grain and yield in maize(Zea may L.)[J].Acta Agronomica Sinica,2007,33(1):38-42.
[31] 吕艳梅,谭伟平,肖层林,范美蓉,廖育林. 高温对优质水稻籽粒淀粉形成及淀粉合成相关酶活性的影响[J]. 华北农学报,2014,29(1):135-139.doi:10.7668/hbnxb.2014.01.025.
Lü Y M,Tan W P,Xiao C L,Fan M R,Liao Y L. Effect of high temperature on starch formation of grain and activities of enzymes related to starch synthesis of quality rice varieties[J]. Acta Agriculturae Boreali-Sinica,2014,29(1):135-139.
[32] 傅晓艺,何明琦,史占良,赵彦坤,王秀堂,郭进考. 灌浆期高温胁迫对小麦灌浆特性和品质的影响[J]. 麦类作物学报,2015,35(6):867-872.doi:10.7606/j.issn.1009-1041.2015.06.20.
F u X Y,He M Q,Shi Z L,Zhao Y K,Wang X T,Guo J K. Effect of high temperature stress during grain filling period on wheat grain filling characteristics and quality[J]. Journal of Triticeae Crops,2015,35(6):867-872.
[33] 牛丽,刘源,于康坷,刘荣花,李潮海,刘天学. 玉米杂交种苗期耐热性评价[J].玉米科学,2015,23(1):107-114.doi:10.13597/j.cnki.maize.science.2015.01.018.
Niu L,Liu Y,Yu K K,Liu R H,Li C H,Liu T X. Evaluation of heat-tolerance of maize hybrids at seedling stage[J]. Journal of Maize Sciences,2015,23(1):107-114.
[34] 张英华,杨佑明,曹莲,郝杨凡,黄菁,李金鹏,姚得秀,王志敏. 灌浆期高温对小麦旗叶与非叶器官光合和抗氧化酶活性的影响[J]. 作物学报,2015,41(1):136-144.doi:10.3734/SP.J.1006.2015.00136.
Zhang Y H,Yang Y M,Cao L,Hao Y F,Huang J,Li J P,Yao D X,Wang Z M. Effect of high temperature on photosynthetic capability and antioxidant enzyme activity of flag leaf and non-leaf organs in wheat[J]. Acta Agronomica Sinica,2015,41(1):136-144.

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