Analysis of miRNA Expression Profile in Young Ear of Two Wheat Varieties Under Low Temperature Stress at the Female Stamen Primordium Differentiation Stage

doi:10.7668/hbnxb.20191253

Abstract

Abstract: To explore the relevant miRNAs and their response laws of wheat varieties with different resistance to cold in spring under the stress of low temperature in spring(late spring cold), high-throughput sequencing(Small RNA-seq) technology was used to analyze the young panicles of Aikang 58(AK58) and Zhengmai 366(ZM366) in the differentiation stage of pistil and stamen primordium at 0℃ low temperature stress for 72 h and without low temperature treatment(control).The purpose of sequencing is provide miRNA expression profiles and information on differentially expressed miRNAs, and reveal the role of differentially expressed miRNAs in wheat resistance to late spring cold. Through bioinformatics analysis, under the condition of padj<0.05 and|log₂(Fold_change)| ≥ 1, the miRNAs with significant differential expression was screened under low temperature stress;using psRobot(v1.2) to predict the differentially expressed miRNA target genes, and then target genes with significantly differentially expressed miRNAs were subjected to GO enrichment analysis and KEGG Pathway analysis. The results showed that 112 types of miRNAs were identified by sequencing of wheat ears, belonging to 38 different MIRNA families.AK58 and ZM366 identified 85 and 88 differentially expressed miRNAs, respectively. AK58 had 27 miRNAs with significant differences in expression, 23 of which were significantly up-regulated, 4 were significantly down-regulated, and 2 miRNAs with extremely significant differential expression were tae-miR9672b, tae-miR9672a-3p;ZM366 had 48 miRNAs with significant differential expression, 13 with significantly up-regulated expression, 35 with significantly down-regulated expression, 4 miRNAs with extremely significant differential expression, tae-miR9672b, tae-miR9672a-3p, tae-miR6201, tae-miR9674b-5p.Target genes were predicted for the differentially expressed miRNAs of AK58 and ZM366 under low temperature stress. 85 differentially expressed miRNAs of AK58 correspond to 848 target genes;88 differentially expressed miRNAs of ZM366 correspond to 6 537 target genes.GO function enrichment analysis results showed that AK58 differentially expressed miRNA target genes had 20 biological processes, 6 cell components, and 6 molecular function categories extremely significantly enriched(FDR<0.01).ZM366 had 10 biological processes, 14 cell components, and 19 molecular functional categories extremely significantly enriched(FDR<0.01).The KEGG metabolic pathway(Pathway) enrichment analysis of the differentially expressed miRNA target genes in the young ears of two wheat varieties showed that AK58 differentially expressed miRNA target genes were significantly enriched(P<0.05) to RNA polymerase, purine metabolism, and pyrimidine metabolism, photosynthetic organism carbon fixation pathway, autophagy, tropane, piperidine and pyridine alkaloid biosynthesis, nucleotide excision repair, mismatch repair, DNA replication, ribosome, niacin and nicotinamide metabolism 11 metabolic pathways.ZM366 differentially expressed miRNA target genes were significantly enriched(P<0.05) in plant pathogen interactions, plant circadian rhythms, degradation of valine, leucine and isoleucine, cell cycle, phenylalanine metabolism, propionic acid biology synthesis, RNA polymerase, plant hormone signal transduction, cyanamide acid metabolism, biosynthesis of secondary metabolites, carbon fixation of photosynthetic organisms, pyruvate metabolism, carotenoid biosynthesis, 13 metabolic pathways.Wheat miRNA regulation of mRNA participates in wheat response to low temperature stress. It is possible that miRNAs such as miRNA171a, miRNA156, miRNA398 and other miRNAs can mediate regulation to resist cold stress. Plant circadian rhythms may be closely related to low temperature stress.The reason for the different resistance to spring cold of the two wheat varieties may be related to the different expression patterns of target genes in the miRNA-regulated photomorphogenesis pathway and flowering process.

Key words: Wheat, Young spike, Low-temperature stress, RNA-seq, miRNA

CLC Number:

S512.03
Q78

ZHANG Ziyang, WANG Bin, WANG Zhiyu, WANG Zhiwei, ZHU Qidi, RU Zhengang, LIU Mingjiu. Analysis of miRNA Expression Profile in Young Ear of Two Wheat Varieties Under Low Temperature Stress at the Female Stamen Primordium Differentiation Stage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 81-94. doi: 10.7668/hbnxb.20191253.

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