Abstract: Heat shock protein 90(Hsp90) gene was cloned and used to reveal the molecular adaption mechanisms of high temperature stress in Stegobium paniceum (Linnaeus). In this study,we tested the survival rate of S. paniceum adults to high temperatures by bioassay method. A full-length cDNA encoding Hsp90 was cloned and characterized by RT-PCR technique. This cDNA was termed as SpHsp90 (GenBank accession No. KX356691),and its expression levels under different high temperatures were detected by using quantitative Real-time PCR(qPCR). The results showed that the survival rate of S. paniceum was significantly decreased as temperature was elevated,and the adults could not survive at 44℃. The open reading frame of SpHsp90 is 2 172 bp and encoding 723 amino acid residues,with a theoretical molecular weight of 82.6 kDa and isoelectric point of 5.01. SpHSP90 had five signature sequences of HSP90 family and a C-terminal cytoplasmic character sequence(MEEVD). The deduced amino acid residues of SpHsp90 had high similarity with Hsp90 proteins from other insect species. The qPCR analysis showed that the expression level of SpHsp90 was significantly increased 2 hours after exposed to 38,40,42℃,compared to the control. The results suggest that the SpHsp90 gene may play an important role in response to high temperature stress in S. paniceum.

Key words: Stegobium paniceum, Heat shock protein, Gene cloning, Expression pattern

CLC Number: 

• Q965

• S435.67

• Q78