Transcriptome Analysis Revealing the Mechanisms of Kernel Dehydration Rate Responding to ABA Application in Maize

doi:10.7668/hbnxb.20190948

Abstract

Abstract: In order to explore the molecular mechanism of kernel dehydration rate changes and to find out the differentially expressed genes(DEGs) and metabolic pathways in DK517 and ZD1002 after spraying exogenous abiscisic acid(ABA) during grain-filling stage,the ear leaves of DK517 (with faster dehydration rate) and ZD1002 (with slower dehydration rate) before and after spraying ABA were used in sequencing with Illumina HiSeq^TM2500 high-throughput sequencing technology. After sequence quality control,genome alignment,sequencing saturation,gene coverage and gene redundant analysis,gene functions were annotated with reference database. The results were as below:1 732 DEGs were detected in DK517-ABA80 VS DK517-CK group,of which 1 251 DEGs were up-regulated and 481 DEGs were down regulated. 52 DEGs were detected in ZD1002-ABA80 VS ZD1002-CK group,24 of which were up regulated. There were 3 867 DEGs,which were detected in DK517-ABA80 VS ZD1002-ABA80,of which 1 946 DEGs were up regulated. The GO analysis,COG analysis and KEGG pathways were all different in the above three control groups.Important transcription factor families were obtained,including AGC protein kinase family,AP2/ERF transcription factor family,ARID transcription factor family,AUX/IAA transcription factor family,Alfin-like transcription factor family,Aur transcription factor family,B3 transcription factor family,BBR-BPC transcription factor family,BES1 transcription factor family,BUB transcription factor family and C2C2-CO-like transcription factor. Genes Zm00001d035000,Zm00001d042063,Zm00001d045314 were all expressing in DK517 and ZD1002. 73 DEGs were related to water metabolism,which played important roles in kernel dehydration acting as downstream genes regulated by exogenous ABA.

Key words: Maize, Ear leaf, Exogenous abscisic acid (ABA), Illumina RNA-sequencing, Differentially expressed genes(DEGs), Kernel dehydration rate

CLC Number:

S513.03
Q78

LI Chuan, HUANG Lu, ZHANG Meiwei, ZHANG Panpan, LIU Jingbao, NIU Jun, QIAO Jiangfang. Transcriptome Analysis Revealing the Mechanisms of Kernel Dehydration Rate Responding to ABA Application in Maize[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 15-26. doi: 10.7668/hbnxb.20190948.

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References

[1] 柳枫贺,王克如,李健,王喜梅,孙亚玲,陈永生,王玉华,韩冬生,李少昆.影响玉米机械收粒质量因素的分析[J].作物杂志,2013(4):116-119.doi:10.16035/j.issn.1001-7283.2013.04.005. Liu F H,Wang K R,Li J,Wang X M,Sun Y L,Chen Y S,Wang Y H,Han D S,Li S K.Factors affecting corn mechanically harvesting grain quality[J]. Crops,2013(4):116-119.
[2] 谢瑞芝,雷晓鹏,王克如,郭银巧,柴宗文,侯鹏,李少昆.黄淮海夏玉米子粒机械收获研究初报[J].作物杂志,2014(2):76-79.doi:10.16035/j.issn.1001-7283.2014.02.001. Xie R Z,Lei X P,Wang K R,Guo Y Q,Chai Z W,Hou P,Li S K.Research on corn mechanically harvesting grain quality in Huanghuaihai Plain[J]. Crops,2014(2):76-79.
[3] 卫晓轶,魏锋,洪德峰,马俊峰,马毅,王稼首,张学舜,闰玉信,刘震宇,胡宁.黄淮海不同生态区玉米机械化粒收初步研究[J].河南农业科学,2019,48(11):40-44.doi:10.15933/j.cnki.1004-268.2019.11.006. Wei X Y,Wei F,Hong D F,Ma J F,Ma Y,Wang J S,Zhang X S,Run Y X,Liu Z Y,Hu N.Preliminary research on maize grain mechanical harvest in different Huang-huai-hai ecological areas[J]. Journal of Henan Agricultural Sciences, 2019,48(11):40-44.
[4] 乔江方,朱卫红,李川,代书桃,张美微,黄璐,牛军,郭国俊,刘京宝.不同密度夏玉米群体冠层特征与籽粒含水率的关系[J].河南农业科学,2018,47(5):12-15,35.doi:10.15933/j.cnki.1004-3268.2018.05.003. Qiao J F,Zhu W H,Li C,Dai S D,Zhang M W,Huang L,Niu J,Guo G J,Liu J B.Relationship between canopy structure and grain moisture content in summer maize with different density[J]. Journal of Henan Agricultral Sciences,2018,47(5):12-15,35.
[5] 郭佳丽,吕志尧,吕颖颖,胡海军,姚晓云,贾森,李凤海,史振声.玉米粒部性状对子粒脱水速率的影响[J].玉米科学,2014,22(4):33-38.doi:10.13597/j.cnki.maize.science.2014.04.033. Guo J L,Lü Z R,Lü Y Y,Hu H J,Yao X Y,Jia S,Li F H,Shi Z S.Effect of kernel characteristics on kernel dehydration rate of maize[J]. Journal of Maize Sciences,2014,22(4):33-38.
[6] 刘艳秋,李明顺,李新海,雍洪军,颜娜,周志强,张晓星,夏仁培,张德贵,郝转芳,翁建峰,白丽,史振声,张世煌.1970s-2000s玉米主栽品种灌浆与脱水速率研究[J].玉米科学,2015,23(1):85-91.doi:10.13597/j.cnki.maize.science.2015.01.014. Liu Y Q,Li M S,Li X H,Yong H J,Yan N,Zhou Z Q,Zhang X X,Xia R P,Zhang D G,Hao Z F,Weng J F,Bai L,Shi Z S,Zhang S H.Preliminary study on grain filling and dehydration rate of maize hybrids used predominantly in 1970s-2000s[J]. Journal of Maize Sciences,2015,23(1):85-91.
[7] 张文杰,王永宏,王克如,赵健,赵如浪,李少昆.不同玉米品种子粒脱水速率研究[J].作物杂志,2016(1):76-81.doi:10.16035/j.issn.1001-7283.2016.01.014. Zhang W J,Wang Y H,Wang K R,Zhao J,Zhao R L,Li S K.Grain dehydration rate of different maize varieties[J]. Crops,2016(1):76-81.
[8] 李璐璐,谢瑞芝,范盼盼,雷晓鹏,王克如,侯鹏,李少昆.郑单958与先玉335子粒脱水特征研究[J].玉米科学,2016,24(2):57-61,71.doi:10.13597/j.cnki.maize.science.20160212. Li L L,Xie R Z,Fan P P,Lei X P,Wang K R,Hou P,Li S K.Study on dehydration in kernel between Zhengdan 958 and Xianyu 335[J]. Journal of Maize Sciences,2016,24(2):57-61,71.
[9] 雷蕾,王威振,方伟,张子学,刘正,李文阳.影响夏玉米生理成熟后子粒脱水的相关因素分析[J].玉米科学,2016,24(3):103-109.doi:10.13597/j.cnki.maize.science.20160317. Lei L,Wang W Z,Fang W,Zhang Z X,Liu Z,Li W Y.Analysis of factors affection the kernel dehydrating after physiological mature in summer maize[J]. Journal of Maize Sciences, 2016,24(3):103-109.
[10] 王克如,李少昆.玉米籽粒脱水速率影响因素分析[J].中国农业科学,2017,50(11):2027-2035.doi:10.3864/j.issn.0578-1752.2017.11.008. Wang K R,Li S K.Analysis of influencing factors on kernel dry-down-rate of maize hybrids[J]. Scientia Agricultura Sinica,2017,50(11):2027-2035.
[11] 李川,乔江方,谷利敏,夏来坤,朱卫红,黄璐,刘京宝.影响玉米籽粒直接机械化收获质量的生物学性状分[J].华北农学报,2015,30(6):164-169.doi:10.7668/hbnxb.2015.06.025. Li C,Qiao J F,Gu L M,Xia L K,Zhu W H,Huang L,Liu J B.Analysis of maize biological traits which affected corn kernel mechanically harvesting qualities[J]. Acta Agriculturae Boreali-Sinica, 2015,30(6):164-169.
[12] 李璐璐,谢瑞芝,王克如,明博,侯鹏,李少昆.黄淮海夏玉米生理成熟期子粒含水率研究[J].作物杂志,2017(2):88-92.doi:10.16035/j.issn.1001-7283.2017.02.015. Li L L,Xie R Z,Wang K R,Ming B,Hou P,Li S K.Kernel moisture content of summer maize at physiological maturity stage in Huanghuaihai region[J]. Crops,2017(2):88-92.
[13] 李川,乔江方,黄璐,张美微,张盼盼,牛军,刘京宝.转录组及代谢组联合解析玉米响应花粒期高温胁迫机制[J].华北农学报,2020,35(1):8-21.doi:10.7668/hbnxb.20190649. Li C,Qiao J F,Huang L,Zhang M W,Zhang P P,Niu J,Liu J B.Transcriptome and metabolome analysis to reveal the mechanisms responding to high temperature stress in anthesis stage of maize[J]. Acta Agriculturae Boreali-Sinica,2020,35(1):8-21.
[14] 李凤海,郭佳丽,于涛,史振声.不同熟期玉米杂交种及其亲本子粒脱水速率的比较研究[J].玉米科学,2012,20(6):17-20,24.doi:10.13597/j.cnki.maize.science.2012.06.006. Li F H,Guo J L,Yu T,Shi Z S.Comparative study on dehydration rate of kernel among maize hybrids and parents with different maturity periods[J]. Journal of Maize Sciences, 2012,20(6):17-20,24.
[15] 崔喜艳,陈众峰,陈展宇.AP2/ERF转录因子对植物非生物胁迫应答的研究进展[J].吉林农业大学学报,2015,37(4):417-423.doi:10.13327/j.jjlau.2015.2565. Cui X Y,Chen Z F,Chen Z Y.Advances of researches on AP2/ERF transcription factor response to plant abiotic stress[J]. Journal of Jilin Agricultural University,2015,37(4):417-423.
[16] 卢丞文,潘晓琪,朱本忠.ERFs转录因子及其在植物胁迫应答中的作用[J].生物技术通报,2012(3):22-27.doi:10.13560/j.cnki.biotech.bull.1985.2012.03.023. Lu C W,Pan X Q,Zhu B Z.ERF transcription factors and their function in plant tolerance to environmental stress[J]. Biotechnology Bulletin,2012(3):22-27.
[17] Gui J S,Zheng S,Liu C,Shen J H,Li J M,Li L G.OsREM4.1 interacts with OsSERKl to coordinate the interlinking between abscisic acid and brassinosteroid signaling in rice[J]. Developmental Cell,2016,38(2):201-213.doi:10.1016/j.devcel.2016.06.011.
[18] King G J,Chanson A H,McCullum E J,Ohme-Takagi M,Byriel K,Hill J M,Martin L,Mylne J S. The Arabidopsis B3 domain protein VERNALIZATIONI(VRN1)is involved in processes essential for development,with structural and mutational studies revealing its DNA-binding surface[J]. Journal of Biological Chemistry,2013,288(5):3198-3207.doi:10.1074/jbc.M112.438572.
[19] Krogan N T,Yin X J,Ckurshumova W,Berleth T.Distinct subclades of Aux/IAA genes are direct targets of ARFS/MP transcriptional regulation[J]. New Phytologist Trust,2014,8(3):474-483.doi:10.1111/nph.12994.
[20] 刘颖慧,董志平.植物B3转录因子的结构和功能[J].分子植物育种,2017,15(5):1868-1873.doi:10.13271/j.mpb.015.001868. Liu Y H,Dong Z P.The function and structure of plant B3 domain transcription factor[J]. Molecular Plant Breeding,2017,15(5):1868-1873.
[21] Çakir B,Kiliçkaya O, Olcay A C.Genome-wide analysis of Aux/IAA genes in Vitis vinifera: cloning and expression profiling of a grape Aux/IAA gene in response to phytohormone and abiotic stresses[J]. Acta Physiologiae Plantarum,2013,35(2):365-377.doi:10.1007/s11738-012-1079-7.
[22] 苏江,郭天玮,季孔庶.马尾松Aux/IAA家族相关基因克隆及初步分析[J].分子植物育种,2015,13(8):1822-1830.doi:10.13271/j.mpb.013.001822. Su J,Guo T W,Ji K S. Cloning and premilinary analysis of Aux/IAA family genes from Pinus massoniana[J]. Molecular Plant Breeding,2015,13(8):1822-1830.
[23] Chen Q F,Ya H Y,Feng Y R,Jiao Z.Expression of the key genes involved in ABA biosynthesis in rice implanted by ion beam[J]. Applied Biochemistry and Biotechnology,2014,173(1):239-247.doi:10.1007/s12010-014-0837-y.
[24] Sripinyowanich S,Klomsakul P,Boonburapong B,Bang yeekhun T, Asami T,Gu H Y,Buaboocha T,Chadchawan S. Exogenous ABA induces salt tolerance in indica rice(Oryza sativa L.):the role of OsP5CS1 and OsP5CR gene expression during salt stress[J]. Environmental and Experimental Botany,2013,86(2):94-105.doi:10.1016/j.envexpbot.2010.01.009.
[25] Li Y L,Dong Y B,Yang M L,Wang Q L,Shi Q L,Zhou Q,Deng F,Ma Z Y,Qiao D H,Xu H.QTL detection for grain water relations and genetic correlations with grain matter accumulation at four stages after pollination in maize[J]. Journal of Plant Biochemistry and Physiology,2014,2(1):1-9.doi:10.4172/2329-9029.1000121.
[26] Wang W S,Zhao X Q,Li M,Huang L Y,Xu J L,Zhang F,Cui Y R,Fu B Y,Li Z K.Complex molecular mechanisms underlying seedling salt tolerance in rice revealed by comparative transcriptome and metabolomic profiling[J]. Journal of Experiment Botany, 2016,67(1):405-419.doi:10.1093/jxb/erv476.
[27] 李恩. AGC蛋白激酶通过磷酸化RopGEFs调控花粉管和根毛的顶端生长[D].泰安:山东农业大学,2017. Li E. AGC kinases regulate the tip growth of pollen tubes and root hairs through phosphorylation of PopGEFs[D].Taian:Shandong Agricultural University,2017.
[28] 王慧,刘洪,杨奕琦,许思思,熊兴耀,周倩.马铃薯AP2/ERF转录因子的克隆及植物表达载体构建[J].分子植物育种,2020,18(8):2563-2568.doi:10.13271/j.mpb.018.002563. Wang H, Liu H,Yang Y Q, Xu S S, Xiong X Y, Zhou Q. Cloning and plant expression vector construction of transcription factors AP2/ERF from potato[J].Molecular Plant Breeding,2020,18(8):2563-2568.
[29] 樊书宏,施海涛,刘国银.一个木薯AUX/IAA转录因子的克隆及调节活性氧功能分析[J].分子植物育种,2019,17(2):362-369.doi:10.13271/j.mpb.017.000362. Fan S H, Shi H T, Liu G Y.Cloning of an AUX/IAA transcription factor in cassava and functional analysis of its role in regulating reactive oxygen[J].Molecular Plant Breeding,2019,17(2):362-369.
[30] 王莲哲,向丽君,李健康,王渊,周帅,李冰冰.小麦B3类转录因子REM-1基因的克隆与表达分析[J].分子植物育种,2019,17(15):4853-4858.doi:10.13271/j.mpb.017.004853. Wang L Z,Xiang L J, Li J K, Wang Y, Zhou S, Li B B.Cloning and expression analysis of B3 group transcription factor REM-1 in wheat[J].Molecular Plant Breeding,2019,17(15):4853-4858.

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