SSR Markers Linked of the Resistance Gene for Downy Mildew in Cucumis melo ssp. melo

doi:10.7668/hbnxb.2016.S1.014

Abstract

Abstract: In order to study the inheritance of melon T115,founding the molecular markers linked to resistance genes and locating the resistance genes.Parental lines and(T115 SP Hongxincui) F₁,F₂,back cross populations BCr and BCs were scored for response to inoculation with[Pseudoperonospora cubensis (Berk.etCurt.) Rostov.].The simple sequence repeat(SSR) technique and bulk segregant analysis(BSA) were used to identify molecular markers linked to the resistance of melon downy mildew.A total 1 090 pairs SSR primers could detect the polymorphism between the resistant pool and susceptible pool which based on the ICuGI constructed genetic linkage map.The resistance to downy mildew in T115 was controlled by one single dominant gene. An unique 120 bp fragment was amplified with the primer DM0073.It was identified that was linked to resistance gene of T115 at a distance of 3.6 cM.The resistance gene was located to LG1.This SSR bands indicated that the marker could be useful for marker assisted selection in melon breeding for downy mildew resistance.

Key words: Cucumis melo ssp. melo, Downy mildew, Disease resistance gene, SSR marker

CLC Number:

Q78
S652.03

ZHANG Xuejun, YANG Yong, LI Meihua, YANG Juntao, YI Hongping. SSR Markers Linked of the Resistance Gene for Downy Mildew in Cucumis melo ssp. melo[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 80-85. doi: 10.7668/hbnxb.2016.S1.014.

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