Establishment of SSR Fingerprint on Melon Hybrids and Their Parents

doi:10.7668/hbnxb.2015.S1.012

Abstract

Abstract: For the purpose of melon identification,SSR technology was used to establish fingerprint for 18 melon accessions included six F₁ hybrids and their parents.Based on 50 SSR primer pairs were assessed validation of the amplification in above melon accessions.Used three primer pairs C17,C21and C30,SSR fingerprint was obtained for six melon F₁ hybrids and their parents,which was able to distinguish all of the 18 accessions.Moreover,primer C21was applied in purity identification of M324 hybrid,the result indicated that the purity was 89%,which were approximate to their field purity(90%).

Key words: Melon, SSR marker, Fingerprint, Purity identification

CLC Number:

S652.03

WANG Jin, YOU Jia, WANG Xi-qiao, WEN Zhao-hui, WANG Jun-ping. Establishment of SSR Fingerprint on Melon Hybrids and Their Parents[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 66-68. doi: 10.7668/hbnxb.2015.S1.012.

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http://www.hbnxb.net/EN/Y2015/V30/IS1/66

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