Physiological Data Processing and Extreme Material Selection of Low Temperature and Low Light Tolerance in Melon

doi:10.7668/hbnxb.20191243

Abstract

Abstract: The chlorophyll content of leave and the malonaldehyde(MDA)content of root were the important physiological index for identification of low temperature and low light in melon. In order to select the extreme material by the correct physiological data processing,eight core parental inbred lines were used to be evaluated. The evaluation results derived from two physiological index and the injury index were compared first. The injury index was calculated according to the injury classification. Two data processing methods were used in physiological data. One was using the measure value of SPAD and MDA after stress directly,another was using the absolute value of SPAD and MDA calculated between before and after stress. The compare results showed that under low temperature and low light stress,the leaf chlorosis and macerating root was happened in melon seedling. The SPAD value of poor tolerance melon inbred lines was getting smaller and the MDA content was getting larger. Using the measure value of SPAD and MDA after stress directly could get the same low temperature and low light stress tolerance results with which identified from injury index. The absolute value of SPAD and MDA calculated between before and after stress could used to evaluate accessorily the sensitive ability to stress for inbred lines. The tolerance inbred line of P143 and the sensitive line of P149 were selected for further research of molecular selection. The results were useful to uniform the low temperature and low light identification in melon and to provide reference for other stress data processing.

Key words: Melon, Low temperature and low light, Stress

CLC Number:

S652.01

SONG Hui, HUANG Yunping, GU Binquan, FU Yujing, ZANG Quanyu, XING Nailin, ZHANG Xiangqin. Physiological Data Processing and Extreme Material Selection of Low Temperature and Low Light Tolerance in Melon[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 183-188. doi: 10.7668/hbnxb.20191243.

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