Effects of NO on Root Physiology and Mineral Content of Watermelon under Aluminum Stress

doi:10.7668/hbnxb.20192297

Abstract

Abstract: To study the alleviating effect of exogenous Nitric oxide(NO) on the root system of watermelon under aluminum stress,watermelon variety Zaojia 84-24 was used as the experimental material,and the effects of different concentrations(50,100,200,500 μmol/L) of NO on the physiological and mineral content of watermelon root under aluminum stress were studied by applying nitroprune(NO donor) under high concentration of aluminum stress(1 200 μmol/L). The results showed that the root electrolyte leakage and the content of malondialdehyde increased under high Al ion stress,and the activities of superoxide dismutase(SOD),peroxidase(POD) and catalase(CAT) were significantly decreased in order to maintain the osmotic equilibrium by significantly increasing the content of proline and soluble sugar in the root. Aluminum stress inhibited the absorption of Ca,Mg,Fe and P,promoted the absorption of K,Zn and Al,but the content of Cu had not changed significantly. After the addition of exogenous NO,the root electrolyte leakage and the content of malondialdehyde decreased,the degree of plasma membrane peroxidation decreased,the content of soluble sugar in the root decreased significantly,and the content of proline increased significantly. The activities of SOD,POD and CAT increased first and then decreased with the increase of NO concentration. NO not only promoted the absorption and accumulation of nitrogen by increasing the activity of nitrate reductase,but also promoted the absorption of Ca,Mg,Fe,Cu and P by watermelon roots,and inhibited the accumulation of Zn,Al and K contents by roots. The addition of L-NAME,an endogenous inhibitor of NO,eliminated the alleviating effect of NO on aluminum stress in watermelon and even inhibited it. These results indicated that exogenous SNP could alleviate oxidative damage of watermelon roots under Al stress,and alleviate the effects of Al toxicity by affecting the absorption of nutrient elements by roots.

Key words: Watermelon, Stress of aluminium, Nitric oxide, Antioxidant enzyme, Minerals

CLC Number:

S651.01

XIAO Jiachang, ZHENG Kaimin, MA Junying, ZHENG Yangxia. Effects of NO on Root Physiology and Mineral Content of Watermelon under Aluminum Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 116-123. doi: 10.7668/hbnxb.20192297.

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