Polymorphisms of MyoG Gene and Analysis of Relevance with Growth Performances in Yak

doi:10.7668/hbnxb.2018.06.015

Abstract

Abstract: The aims of this study were to compare the characteristics of maiwa yak in Sichuan, leiwuqi yak, shenzhayak, pali yak, sibu yak in Tibet, select the MyoG gene to detection of SNPs, to reveal the association between different genotypes and growth traits. This study provided a reference for the effective evaluation of the growth performance to the main yak population in Tibet. The DNA pool technology and direct sequencing were adopted to study in the genetic variation of the candidate genes. GLM statistical model of SPSS 17.0 software was used to analyze the association with different genotypes corresponding to the SNPs MyoG with growth traits, such as body height, body length, chest circumference, cannon circumference and body weight. The results showed that the growth indexes of shenzha yak were significantly lower than maiwa yak, leiwuqi yak and pali yak, but the traits of leiwuqi yak were better. Different ecological environments and climatic conditions directed affect grassland, vegetation types and their growth conditions, which were affect the feed intake and growth of yak. Four SNPs were found in MyoG gene (g.757 T>C,g.662 G>A,g. 539 A>G and g. 2216 A>G). There were three genotypes. The fitness test showed that MyoG gene on polymorphic loci in Hardy-Weinberg equilibrium state. The polymorphism of g.757 T>C,g.662 G>A and g. 539 A>G was higher in shenzha yak and pali yak, and lower in other groups. The different significance test indicated that the g.757 T>C,g.662 G>A and g. 539 A>G of the MyoG gene were significantly related to the body height(P<0.05). There were the major effect genes influencing the body height of the yak or loci might be closely linked to the major effect gene and they could be regarded as the genetic markers for the assistant selection.

Key words: Yak, MyoG gene, Polymorphism, Growth traits, Association analysis

CLC Number:

S823
S813.3

CHAI Zhixin, XIN Jinwei, WANG Hui, GUO Lin, ZHONG Jincheng, ZHANG Chengfu, JI Qiumei. Polymorphisms of MyoG Gene and Analysis of Relevance with Growth Performances in Yak[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(6): 108-115. doi: 10.7668/hbnxb.2018.06.015.

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