Expression of SiCCT Gene and Its Association Analysis with Main Agronomic Traits in Foxtail Millet

doi:10.7668/hbnxb.20192054

Abstract

Abstract: In order to reveal the possible functional effects of SiCCT gene, and provide foundation for further research of the function of SiCCT gene in photo-thermal interaction-controlled flowering process of foxtail millet, the photoperiod sensitive millet variety Huangmaogu was used as experimental material to analyze the diurnal expression patterns of SiCCT gene under long-day and high temperature(LD, 27℃), long-day and low temperature(LD, 22℃), short-day and high temperature(SD, 27℃), short-day and low temperature(SD, 22℃), totally four photo-thermal combinational conditions by fluorescence quantitative PCR. Furthermore, based on the survey data of ten main agronomic traits for two consecutive years, candidate gene-based association analysis was performed. The results showed that the expression of SiCCT gene was not affected by temperature, and the expression level of SiCCT gene under long-day was wholly higher than that under short-day at light period. While at darkness period, the expression of SiCCT gene was affected by temperature, especially under short-day, the expression level of SiCCT gene at low temperature was obviously higher than that at high temperature. Based on the association analysis of candidate gene, it was found that 11 polymorphic loci detected in SiCCT gene were significantly associated with 8 major agronomic traits(P <0.05), among which, SNP-10 was associated with heading stage, leaf number and grain weight per panicle at Luoyang, Henan and Jilin, Jilin, SNP-100 and SNP-104 were associated with plant height, leaf number and panicle length at Luoyang. SNP-51 was detected at Ledong of Hainan, Luoyang and Jilin for two consecutive years, but the related traits were different, which were ear diameter, branch number per panicle and leaf number at Ledong, Luoyang and Jilin, respectively.The expression of SiCCT gene was controlled by photoperiod, and temperature also affected its expression at darkness period under short-day. SiCCT gene possessed pleiotropic effects, but its function was regulated by photoperiod condition.

Key words: Setaria italica, Photoperiod, Temperature, SiCCT gene, Agronomic trait, Association analysis

CLC Number:

S515

WANG Zhenshan, YUAN Xilei, YAN Liuyan, YANG Pengjuan, SANG Luman, JIA Xiaoping, BAI Junyan. Expression of SiCCT Gene and Its Association Analysis with Main Agronomic Traits in Foxtail Millet[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 43-49. doi: 10.7668/hbnxb.20192054.

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