Analysis of HMW-GS and Translocation Line in Progenies of Doubled Haploid from Wheat×Maize Cross

doi:10.7668/hbnxb.2016.04.021

Abstract

Abstract: To improve the efficiency of high quality wheat breeding program,clarify the composition of HMW-GS and Gliadin in the early generation,296 lines from the wheat maize cross were analyzed useing SDS-PAGE method in this experiment,along with this Gliadin were analyzed useing A-PAGE method.A statistics showed that female parent Heng 09-6324 carried null,7+9,2+12 subunits,and were 1BL/1RS translocation lines with Sec-1 characteristic band.Male parent Shiluan 02-1 carried 1,7+9,5+10 subunits,and were non 1BL/1RS translocation.Six types of HMW-GS all originated from parents were identified from 296 DH lines.The frequency of subunits 5+10 which positively related to bread baking quality was 35.13%.A statistics showed that in the A-PAGE the type of translocation of the DH lines accounted for 54.05%,and the percent of lines with subunits 1 and 5+10,but non Sec-1 translocation were 15.20%,these lines could be used as new germplasm for quality breeding.The paper indicated that that the method of half-seed SDS-PAGE and A-PAGE which could be used to identify and select excellent plants in early generation breeding effectively.

Key words: Wheat, Maize, Doubled haploid, Half-seed electrophoresis, HMW-GS, Secalin

CLC Number:

PANG Jianzhou, WANG Xuezheng, QIAN Xiaozhe, WANG Chenyang, CHEN Shuping. Analysis of HMW-GS and Translocation Line in Progenies of Doubled Haploid from Wheat×Maize Cross[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(4): 126-129. doi: 10.7668/hbnxb.2016.04.021.

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References

[1] 陈新民,陈孝.小麦×玉米产生单倍体及双单倍体研究进展[J].麦类作物学报,1998,18(3):4-7.
[2] 李春莲,薛芳,靳凤,等.等普通小麦DH群体条锈病抗性鉴定及抗病基因的分子检测[J].麦类作物学报,2013,33(6)：1300-1304.
[3] Barclay I R.High frequencies of haploid production in wheat (Triticum aestivum L.) by chromosome elimination[J].Nature,1975,256:410-411.
[4] Zenkteler M,Nitzsche W.Wide hybridization experiments in cereals[J].Theor Appl Genet,1984,68:311-315.
[5] Laurie D A,Bennett M D.Wheat maize hybridization[J].Theor Appl Genet,1986,28:313-316.
[6] Suenaga K,Nakajima K.Efficient production of haploid wheat (Triticum aestivum) through crosses between Japanese wheat and maize (Zea mays)[J].Plant Cell Reports,1989,8(5):263-266.
[7] Depauw R M,Knox R E,Humphreys D G,et al.New breeding tools impact Canadian commercial farmer fields[J].Czech Journal of Genetics and Plant Breeding,2011,47(SI):S28-S34.
[8] 陈新民,何中虎,刘春来,等.利用小麦×玉米诱导单倍体技术育成小麦新品种中麦533[J].麦类作物学报,2011,31(3):427-429.
[9] Chen X M,Wang F J,Li S M,et al.Stable production of wheat haploid and doubled haploid by wheat×maize cross[J].Acta Agronomica Siniac,2013,39(12):1-6.
[10] 封德顺.体细胞杂种小麦与供体高冰草的高分子量麦谷蛋白亚基及其基因分析[D].济南:山东大学,2004:23-26.
[11] 赵庆玮,王洪刚.国内外部分小麦种质资源高分子量麦谷蛋白亚基组成分析[J].山东农业科学,2007(6):25-28.
[12] 刘丽,周阳,何中虎,等.高、低分子量麦谷蛋白亚基等位变异对小麦加工品质性状的影响[J].中国农业科学,2004,37(1):8-14.
[13] Cooke R J.The classification of wheat cultivars using a standard reference electrophoresis method[J].National Association of Colleges and Teachers of Agriculture,1987,17:273-281.
[14] Jazaeri S,Bock J E,Bagagli M P,et al.Structural modifications of gluten proteins in strong and weak wheat dough during mixing[J].Cereal Chemistry,2015,92(1): 105-113.
[15] 魏益民,张波,关二旗,等.中国冬小麦品质改良研究进展[J].中国农业科学2013,46(20):4189-4196.
[16] Zhou Y,He Z H,Zhang G S,et al.Utilization of 1BL/1RS translocation in wheat breeding in China[J].Acta Agronomica Sinica,2004,30(6):531-535.
[17] 张影全,张晓科,魏益民,等.高分子量麦谷蛋白亚基对小麦蛋白质品质特性的影响[J].西北农业学报,2013,22(1):48-53.
[18] 何中虎,夏先春,罗晶,等.国际小麦育种研究趋势分析[J].麦类作物学报,2006,26(2):154-156.
[19] Ravi m,Chan S W.Haploid plants produced by centromere-mediated genome elimination[J].Nature,2010,464(7288):615-618.
[20] 赵德辉,阎俊,黄玉莲,等.1BL/1RS易位对小麦贮藏蛋白组分含量和面团流变学特性的影响[J].作物学报,2015,41(11):1648-1656.
[21] 魏学军,张娜,胡亚亚,等.53个小麦品种中1BL/1RS易位系的分子检测[J].分子植物育种,2013,11,(4):503-508.
[22] 闫贵云,左静静,冯瑞云,等.玉米花粉诱导小麦单倍体技术研究进展[J].山西农业科学,2015,43(9):1204-1206.

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