Clone of Gene CmKexin of Cordyceps militaris and Its Detection of Expression in Mycelium

doi:10.7668/hbnxb.2016.04.019

Abstract

Abstract: In order to study the characteristics of subtilisin-like protease expression in the mycelia of fungus Cordyceps militaris,CmKexin gene ORF sequences of C.militaris was got by RT-PCR,then CmKexin sequence was analysed.The Real-time PCR and Northern Blotting were used to the CmKexin gene expression analysis in the C.militaris mycelia in blue light irradiation of different time.Results showed the CmKexin gene ORF sequence was obtained from C.militaris mycelia.The bioinformatics analysis for the translation products of CmKexin gene showed that there were 1 peptidase S8 family domain of protein convertases (143-429) and 1 proprotein convertase P-domain(514-600) in the protein.The two domains existed in CmKexin demonstrate the characteristics of subtilisin-like protease.Under the dark pre-culture in 96 h,a large amounts of transcript of CmKexin gene in the mycelia could be detected by Northern Blotting and Real-time PCR in the continuous blue light irradiation to 48-50 hours.But the transcription of CmKexin gene wasn't detected in the other time of C.militaris mycelia culture.These results would provide the basis for using the subtilisin-like protease in Cordyceps militaris.

Key words: Cordyceps militaris, CmKexin gene, Subtilisin-like protease, Northern Blotting, Real-time PCR

CLC Number:

MAO Yumei, LI Qin, FU Mingjia, JIN Huayan, SHEN Junliang, ZHONG Xueqing. Clone of Gene CmKexin of Cordyceps militaris and Its Detection of Expression in Mycelium[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(4): 112-118. doi: 10.7668/hbnxb.2016.04.019.

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