Cloning and Expression Analysis of Ent-Kaurene Synthasegene MdKS in Apple(Malus domestica Borkh)

doi:10.3969/j.issn.1000-7091.2013.02.009

Abstract

Abstract: Ent-kaurene synthase(KS) is a critical enzyme in the pathway ofgibberellins biosynthesis. In this research,ent-kaurene synthasegene in columnar apple,designated as MdKS,was isolated from apple(Malus domes-tica Borkh)stem apical tissue by homologous cloning technology. The full-length cDNA consisted of 2 214 nucleo-tides encoding a putative protein of 737 amino acids. MdKS contained a core functional domain DDXXD of plant class terpene synthase. Amino acids cluster analysis showed that KS from apple was highly homologous with PpKS firstly,had 98% similarity,and followed by that from Castanea sativa,had 73% similarity. The Real-time quantita-tive PCR analysis showed that MdKSgene were always expressed in columnar and standard apple trees during thegrowing season. In the earlygrowing season,the MdKS of standard apples were expressed at higher level than that of columnar apples,but on early June,it was opposite.

Key words: Apple, Ent-kaurene synthase, Dwarf, Real-time PCR

CLC Number:

S661. 1
Q78

DENG Xiao-yun, DAI Hong-yi, LIANG Mei-xia. Cloning and Expression Analysis of Ent-Kaurene Synthasegene MdKS in Apple(Malus domestica Borkh)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(2): 46-51. doi: 10.3969/j.issn.1000-7091.2013.02.009.

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