Sequence Characters Analysis of a β-Tubulin Gene and Its Promoter in Apple

doi:10.7668/hbnxb.2018.03.013

Abstract

Abstract: As the basic component of plant microtubules,tubulins are of great significance to the formation of cytoskeleton. To investigate the effect of one member of the β-tubulin gene family with the transcript number of MDP0000749824.1 on the stem growth,the coding sequence of this gene was cloned from the apple young shoot tips of standard apple variety Yanfu 3 and the columnar variety Telemon,respectively. This sequence was 1 332 bp in length which encoded a peptide with 443 amino acids. The results of DNAMAN alignment showed that the coding sequence of the gene was identical between Yanfu 3 and Telemon.Using DNA of Yanfu 3 and Telemon as templates,1 661 bp nucleotide sequences was obtained by PCR amplification with promoter specific primers. There were six bases differences between them. Promoter sequence analysis revealed that some hormone responsive elements of gibberellin,abscisic acid,methyl jasmonate and salicylic acid existed in it. Cam35S-MDP0000749824-GFP recombinant vector was constructed. Subcellular localization analysis with tobacco leaves indicated that the protein encoded by MDP0000749824.1 was on the cell membrane and microtubules. qRT-PCR detected that the transcript level of this gene in apical stems of columnar apple Telemon was significantly lower than that of standard apple Yanfu 3. It was speculated that the gene could affect the stem elongation be cause its expression level was regulated by hormones.

Key words: Apple, Tubulin gene, Subcellular localization, Gene expression, Promoter

CLC Number:

S661.03
Q78

ZHANG Haiyue, XIAO Yuxiong, TIAN Yike, WANG Caihong, YANG Shaolan, LI Yu, LI Yuqi. Sequence Characters Analysis of a β-Tubulin Gene and Its Promoter in Apple[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(3): 78-85. doi: 10.7668/hbnxb.2018.03.013.

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