Detection of Japanese Cephalitis Virus from Wild Boars by TaqMan-based Real Time PCR

doi:10.7668/hbnxb.2015.S1.064

ACTA AGRICULTURAE BOREALI-SINICA ›› 2015, Vol. 30 ›› Issue (S1): 355-359. doi: 10.7668/hbnxb.2015.S1.064

Detection of Japanese Cephalitis Virus from Wild Boars by TaqMan-based Real Time PCR

ZHAO Shan¹, LIN Hua², CHEN Shi-jie², YANG Miao², XUE Chang-hua², HAO Zhong-xiang¹, WANG Bin¹, WEN Cai-fang¹, YAN Qi-gui^1,3

1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Sichuan Enter-Exit Inspection and Quarantine Bureau, Chengdu 610041, China;
3. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China

Received:2015-09-10 Published:2015-12-28

Abstract

Abstract: The Japanese cephalitis virus of domesticated boars was detected by the method of TaqMan-based Real time PCR in order to protect wild boars more effectively and assess its security as germplasm resources and risk as domestication.The results showed that the sensitivity of TaqMan-based Real time PCR was 100 times than PCR and the test time reduced by almost 1/2.The coefficient variation(CV) of intra/inter-assay for the same sample was less than 2%.Further detection of the 40 blood samples of domesticated boars,the positive rate of TaqMan-based Real time PCR was 10%,more sensitive than RT-PCR which it was 5%.The results proved that the method could support Japanese cephalitis detection and molecular epidemiological investigation,which also could lay the foundation for eradicating Japanese cephalitis from wild boars.

Key words: Wild boars, Japanese encephalitis virus, TaqMan-based Real-time PCR

CLC Number:

ZHAO Shan, LIN Hua, CHEN Shi-jie, YANG Miao, XUE Chang-hua, HAO Zhong-xiang, WANG Bin, WEN Cai-fang, YAN Qi-gui. Detection of Japanese Cephalitis Virus from Wild Boars by TaqMan-based Real Time PCR[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 355-359. doi: 10.7668/hbnxb.2015.S1.064.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2015.S1.064

http://www.hbnxb.net/EN/Y2015/V30/IS1/355

References

[1] 姚永芳,徐怀亮,杨晓军.野猪遗传学研究进展[J].经济动物学报,2007,11(2):112-115.
[2] 朱洪强,葛志勇,常素慧,等.黄泥河自然保护区野猪冬季栖息地利用[J].生态学杂志,2011,30(4):734-738.
[3] 孙博兴,赵志辉,郭淑艳.野猪种源生态与杂交利用的研究进展[J].四川动物,2009,28(1):153-156.
[4] 周绍春,张海明,孙海义,等.完达山东部林区野猪种群数量和栖息地特征的初步分析[J].兽类学报,2010,30(1):28-34.
[5] 刘鹤,李乐,马强,等.野猪研究进展[J].四川动物,2011,30(2):310-314.
[6] 王宪龙,尹逊河,武道留,等.长白山野猪与莱芜猪杂交一代猪的肉品品质[J].中国兽医学报,2012,32(8):1244-1248.
[7] Fan J M,Luo J,Zhang G P,et al.Identification and characterization of Japanese encephalitis virus envelope protein gene from swine[J].Letters in Applied Microbiology,2010,51(1):11-17.
[8] Vicente J,León-Vizcaíno L,Gortázar C,et al.Antibodies to selected viral and bacterial pathogens in European wild boars from southcentral Spain[J].Journal of Wildlife Diseases,2002,38(3):649-652.
[9] Meng X J,Lindsay D S,Sriranganathan N.Wild boars as sources for infectious diseases in livestock and humans[J].Biological Sciences,2009,364(1530):2697-2707.
[10] Gibbs E P.The public health risks associated with wild and feral swine[J].Revue Scientifique et Technique,1997,16(2):594-598.
[11] 李忠秋,马建章.野猪的遗传学研究与开发利用进展[J].东北农业大学学报,2010,41(1):153-156.
[12] 常洪涛,刘慧敏,赵军,等.家养野猪脑心肌炎病毒的分离、鉴定及全基因组序列分析[J].中国人兽共患病学报,2014(8):793-796.
[13] Patz J A,Epstein P R,Burke T A,et al.Global climate change and emerging infectious diseases[J].Journal of the American Medical Association,1996,275(3):217-223.
[14] 李国栋,张俊华,焦耿军,等.气候变化对传染病爆发流行的影响研究进展[J].生态学报,2013,33(21):6762-6773.
[15] 陶三菊.流行性乙型脑炎的流行监测及预防[J].中国计划免疫,2002,8(4):226-230.
[16] Santhosh S R,Parida M M,Dash P K,et al.Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantitation of Japanese encephalitis virus[J].Journal of Virological Methods,2007,143(1):73-80.
[17] 王帅涛,王新卫,陈陆,等.乙型脑炎病毒荧光定量PCR的建立及对病毒体外复制动态的研究[J].中国兽医学报,2013,33(6):867-873.
[18] Chen Z,Liao Y,Ke X,et al.Comparison of reverse transcription loop-mediated isothermal amplification,conventional PCR and real-time PCR assays for Japanese encephalitis virus[J].Molecular Biology Reports,2011,38(6):4063-4070.
[19] 韦天超,田志军,周艳君,等.猪IFN-γ mRNA TaqMan荧光定量RT-PCR检测方法的建立[J].中国兽医科学,2009,39(2):168-172.

Please choose a citation manager

Content to export

Detection of Japanese Cephalitis Virus from Wild Boars by TaqMan-based Real Time PCR

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 1

Recommended Articles

Metrics

Comments

模态框（Modal）标题

Please choose a citation manager

Content to export

Detection of Japanese Cephalitis Virus from Wild Boars by TaqMan-based Real Time PCR

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 1

Recommended Articles

Metrics

Comments