Cloning and ExpRession of Enzyme Genes Related to Lignin Biosynthesis in the PeRicaRp of Russet Mutant of Dangshansuli

doi:10.7668/hbnxb.2013.06.015

Abstract

Abstract: To study the formation mechanism of the russet mutant of Dangshansuli, the enzyme genes related to the lignin biosynthesis, PAL2,4CL1,CAD1,PPO1,POD4 were cloned and the relative expressions of different genes in the pericarp of Dangshansuli and its russet mutant were analyzed with real -time PCR at 25, 50, 75, 100, 125, 150 and 175 days after full bloom ( DAFB).The results showed that the relative expressions of PAL2,4CL1,CAD1, POD4 and PPO1 genes in pericarp of Xiusu pear were all higher than that of Dangshansuli pear at 25, 50, 75, 100, 125,150 and 175 DAFB.The correlation analysis between the lignin increment and the relative expressions of POD4 enzyme gene exhibited extremely significant differences in the pericarp of Dangshansuli, while that of PAL2, 4 CL1, CAD1 and POD4 enzyme gene in the pericarp of Xiusu.It is believed that PAL2,4CL1,CAD1 and POD4 enzyme genes in biosynthesis of lignin involved in the formation of Xiusu russet skin.

Key words: PyRus, Russet mutant, Gene clone, Real-time PCR

CLC Number:

S661.2

LIU Li, SUN Hong-li, CHENG Zhao-yang, JIA Bing, LIU Pu, YE Zhen-feng, ZHU Li-wu, HENG Wei. Cloning and ExpRession of Enzyme Genes Related to Lignin Biosynthesis in the PeRicaRp of Russet Mutant of Dangshansuli[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 88-92. doi: 10.7668/hbnxb.2013.06.015.

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