Cloning and Expression Characteristics of Solanum tuberosum StPR1 Gene

doi:10.7668/hbnxb.201751433

Abstract

Abstract: In order to explore the correlation between pathogenesis related protein (PR protein) and potato disease resistance, the ability to resist soft rot, bacterial wilt and dry rot in potato was tested. In the previous potato disease resistance study, 17 family gene-binding transcriptome data of PRs were analyzed and the genes were screened, and PR1 was finally determined. The potato Atlantic variety was selected as the material, which was provided by Heilongjiang Academy of Agricultural Sciences. The StPR1 gene was cloned and analyzed by bioinformatics. F.sambucinum and F.avenaceum were used. And E.carotovora subsp. Carotovora Borgey (Ecc), E.chrysanthemi Burkholder. Atroseptica Dye (Ech), can cause soft rot and bacterial wilt. The specific analysis of StPR1 gene expression was carried out after inoculation of potato tuber with E. carotovora subsp. Mc Fadden et Dimock (Eca) and Ralstonia solanacearum (RS). The results showed that the StPR1 gene was 540 bp in length and encoded 179 amino acids. The hydrophobicity and hydrophilicity of the protein are predicted.The GRAV value of the PR1 protein sequence was between +4 and-3, containing hydrophilic and hydrophobic regions, and protein secondary structure. It is predicted to be a mixed protein. The tertiary structure of the protein is predicted to be a tightly complex helical structure with a SCP_PR-1_like conserved domain, which is highly similar to the pepper PR1 gene on an evolutionary branch. The results of qRT-PCR showed that the expression level of StPR1 gene was higher than that of bacterial stress in antifungal stress, and its ability to resist dry rot was better than that against soft rot and bacterial wilt. In summary, when the potato is attacked by external pathogens, StPR1 plays an important role in the disease resistance of the potato.In summary, when the potato is attacked by external pathogens, StPR1 plays an important role in the disease resistance of the potato.

Key words: Solanum tuberosum, StPR1, Gene clone, Expression characteristics, Disease resistance

CLC Number:

S632.03
Q78

LI Xiuyu, HE Fumeng, HAN Yingqi, ZHAO Xiaocan, WU Jiawen, ZHU Yuanfang, ZHOU Lei, SHI Qihai, FENG Zhe, LI Fenglan. Cloning and Expression Characteristics of Solanum tuberosum StPR1 Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 66-71. doi: 10.7668/hbnxb.201751433.

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