Identification and Expression Analysis of Whirly Gene Family in Capsicum annuum L.

doi:10.7668/hbnxb.201750777

Abstract

Abstract: To preliminarily explore the functions and evolutionary relations of Whirly gene family members, the bioinformatics was used to analyze the gene structure, conserved motif, evolutionary relationship and expression patterns, etc., and the fluorescence quantitative PCR was used to determine the expression levels under abscisic acid, high temperature, low temperature and epidemic stress. The results showed that two Whirly genes (CaWHY1 and CaWHY2) were identified from the pepper variety CM334. Compared with the physicochemical properties of other species, Whirly, the CaWHY1 and CaWHY2 have little difference in structure and similarity.The CaWHY2 gene had exactly the same genetic structure with the SlWHY2 gene of tomato, which were clustered together in phylogenetic tree. CaWHY1 and SlWHY1 were also clustered together, but their genetic structures were different, indicating that the structure of the Whirly protein is conserved during the evolution.The expression pattern analysis showed that both CaWHY1 and CaWHY2 could express in pepper CM334, but their expression levels were different in different tissues and at different fruit development stages. CaWHY1 and CaWHY2 were induced to different degrees under different stress treatments, among which CaWHY1 was obviously induced by epidemic diseases. The expression of CaWHY2 under low temperature and abscisic acid stress showed an opposite trend. Above results suggested that, Whirly gene family played a regulatory role in pepper growth and development, and also played a certaun role in various stress.

Key words: Pepper, Whirly gene family, Genomic identification, Stress, Expression analysis

CLC Number:

S641.03
Q78

LÜ Yanyan, WEI Xiaochun, ZHAO Yanyan, YUAN Yuxiang, WANG Zhiyong, YANG Shuangjuan, ZHENG Xiaolan, JIANG Jun, LI Yan, ZHANG Mengyuan, YAO Qiuju, ZHANG Qiang, ZHANG Xiaowei. Identification and Expression Analysis of Whirly Gene Family in Capsicum annuum L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 72-78. doi: 10.7668/hbnxb.201750777.

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