Cloning and Expression Analysis of Ethylene Receptor Gene SlETR6 in Solanum lycopersicum under Abiotic Stress

doi:10.7668/hbnxb.201751117

Abstract

Abstract: In order to identify the function of SlETR6 gene in tomato under different abiotic stresses, the SlETR6 gene was cloned by RT-PCR from Solanum lycopersicum, and the phylogenetic tree was clustered based on its amino acids by MEGA 5.0 soft. The temporal and spatial (included root, leaf, flower, and fruit in different stage) expression patterns of SlETR6 were analyzed by Real-time quantitative PCR (qPCR), and the relative expression level of SlETR6 gene was analyzed by qPCR under high salt stress, high temperature, low temperature and drought stress during tomato seedling development. The results showed that SlETR6 contained 2 265 bp ORFs, encoded 754 amino acid residues. The protein SlETR6 with a molecular mass of 80.05 ku and isoelectric point of 7.28, and had a closer relationship with Solanum tuberosum ETR2-like. Promoter analysis revealed that SlETR6 contained several stress-responsive elements involved in heat, drought, anaerobic, light, ethylene, salicylic acid, and gibberellin signaling. qPCR analysis showed that the SlETR6 was constitutively expressed in all examined tissues, including seed, root, flower, leaf and fruit,and it showed a higher level in flower and breaker fruit. SlETR6 was up-regulated under high salt stress 6 h compare to control, and the expression level was the highest at 12 h under stress, then return to the normal. The transcription level of SlETR6 was up-regulated significantly under high-temperature stress. Drought stress could significantly up-regulated the expression of SlETR6 in the early stage, and the expression level was the highest at 1 h under stress. These results suggested that SlETR6 might function as an stress-responsive gene under high salt stress, high temperature and drought stress during tomato development. This study provided a potential novel candidate for abiotic research in tomato.

Key words: Tomato, SlETR6, Gene clone, Abiotic stress, Expression analysis

CLC Number:

Q78
S641.03

SU Liyan. Cloning and Expression Analysis of Ethylene Receptor Gene SlETR6 in Solanum lycopersicum under Abiotic Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 19-25. doi: 10.7668/hbnxb.201751117.

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