Cloning and Expression Pattern Analysis of PsSPL3 in Tree Peony

doi:10.7668/hbnxb.2017.04.003

Abstract

Abstract: Transcription factor of SPL(SQUAMOSA promoter-binding protein-like) plays very important role among many physiological processes of plants.In order to study the mechanism of action,a 1 057 bp full-length cDNA of PsSPL3 gene was isolated from Paeonia suffruticosa using RACE method.Bioinformatic analysis showed that the ORF length of PsSPL3 was 549 bp which encodes 182 amino acid residues with relative molecular mass of 20.349 4 kDa and the isoelectric point of 9.62.Blast analysis showed that PsSPL3 contained a typical SBP-box structure domain.Evolutionary tree analysis revealed that the putative SPL protein of tree peony and AtSPL3 were clustered in same group,so the SPL gene of tree peony was named PsSPL3. The homology of amino acid compared with PsSPL3 and other known SPL3 was 47.98%-69.46%,and the homology with BpSPL3 was highest with 69.46%.The expression pattern of PsSPL3 in different tissues at the early stage of flowering was analyzed by quantitative Real-time PCR(qPCR),and the results showed that the transcript of PsSPL3 in the root was the highest,and the lower were in stem and petal.The expression pattern of PsSPL3 during dormancy release of tree pony showed that the expression of PsSPL3 gene was first increased and then decreased.When the tree peony flower bud were exposed 14 d chilling,the expression level of PsSPL3 gene was the highest.The expression of PsSPL3 gene in the flower buds after 7 d chilling with exogenous application of GA₃ was significantly higher than that in the control,suggested that the expression of PsSPL3 gene was induced by GA₃ to promote the process of dormancy release in tree peony flower bud.

Key words: Paeonia suffruticosa, PsSPL3, RACE, Real-timePCR, Expression pattern

CLC Number:

Q78
S685.03

ZHAN Xinmei, GUAN Shiming, ZHANG Yuxi. Cloning and Expression Pattern Analysis of PsSPL3 in Tree Peony[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(4): 13-18. doi: 10.7668/hbnxb.2017.04.003.

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