Abstract:
Spraying NaCl solution combined with honeybee pollination was used to overcome self-incompatibility in parent lines of Brassica pekinensis. NaCl solution at different concentrations was tested which revealed that 3% was the optimum concentration. Plants at flowering stage were treated with 3% NaCl solution at different hours of the day, and the most favorable result was achieved in those plants sprayed at 9: 30 am. Seed setting was observed in all the plants manually pollinated at different hours of the day following treatment of 3% NaCl solution. The pollination efficiency of the NaCl treated plants was better than that of the control. Under normal conditions, it took at least 4 days to ensure pollens on the bee body to lose vigor before the bees could be used for pollination. the amount of callose in surface cells of the stigma and the incompatibility after treatment with NaCl solution tended to change in the same direction.
Key words:
Brassica Pekinensis,
NaCl solution,
Self-incompatibility,
Parent line reproduction
CLC Number:
Yang Rui, Yu Yangjun, Xu Jiabing, Chen Guang, Zhang Fenglan, Sun Jizhi, Sun Tianshui. Studies on Techniques of Spraying NaCl Solution on Flowers Combined with Honeybee Pollination to Overcome Self-incompatibility of Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1995, 10(2): 77-81. doi: 10.3321/j.issn:1000-7091.1995.02.015.