Inheritance of Resistance and Screening of Molecular Markers for Turnip Mosaic Virus in Chinese Cabbage

doi:10.7668/hbnxb.2014.02.014

Abstract

Abstract: In order to research the inheritance of TuMV resistance of Chinese cabbage (Brassica campestris L. ssp. Pekinensis; genome AA,2n = 20) and screening molecular markers for related gene, we selected resistant ma- terial 73 and susceptible material 06-247 as parents respectively, and constructed the BC₁ (F₁ × P₁ ) generation. The above generations were inoculated with TuMV-C4 virus mechanically and TuMV resistance was tested by using the method of phenotype observation. We adopted BSA(Bulked segregation analysis) method to screening molecular markers linked to TuMV resistance gene. It has been confirmed that in the group of 73 × 06-247, TuMV resistance was controlled by one pair of recessive genes and three molecular markers named BrID90143 (4. 2 cM) and BrSSR4068(4. 2 cM) and BrID10645 (10. 1 cM), which was laid the foundation for breeding of TuMV resistant Chinese cabbage and further mapping this gene.

Key words: Chinese cabbage, TuMV resistant gene, Molecular markers

CLC Number:

S634.03

ZENG Qiang, ZHANG Zhi-gang, ZHAO Zhi-zhong, LIU Shuan-tao, PEI Yu-he, LIU Xian-xian, XU Wen-ling, REN Ying, SONG Xi-yun, LI Qiao-yun. Inheritance of Resistance and Screening of Molecular Markers for Turnip Mosaic Virus in Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(2): 75-79. doi: 10.7668/hbnxb.2014.02.014.

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