Functional Analysis of a Gene Related to Sclerotia Formation in Botrytis cinerea

doi:10.7668/hbnxb.2014.02.015

Abstract

Abstract: One sclerotia-defect mutant of Botrytis cinerea BMH174 was obtained by Agrobacterium tumefaciensmediated method. The flanking sequence was acquired by TAIL-PCR, and then the T-DNA insertion gene confirmed by Blast in the B. cinerea gene database. The mutant gene was identified BC1G_06945. 1 gene by molecular biolo- gy. The sclerotia formation and pathogenicity in B. cinerea which will facilitate to understand molecular mechanism of conidium development, sclerotia formation and pathogenic in B. cinerea.

Key words: Botrytis cinerea, T-DNA insertional mutant, TAIL-PCR

CLC Number:

S436.631

SI He-long, ZHAO Bin, ZHAO Fu-xin, XING Ji-hong, HAN Jian-min, DONG Jin-gao. Functional Analysis of a Gene Related to Sclerotia Formation in Botrytis cinerea[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(2): 80-84. doi: 10.7668/hbnxb.2014.02.015.

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