Analysis of the Relationship Between BcKMO Gene and MAPK Signaling Pathway in Botrytis cinerea

doi:10.7668/hbnxb.2018.05.012

Abstract

Abstract: To analyzed the relationship between BcKMO gene and MAPK signaling pathway in Botrytis cinerea,the specific inhibitor of MAPK signaling pathways U0126 was used to detect the sensitivity of the BcKMO gene mutants. It was found that the sensitivity of the BCG183 mutant to U0126 was remarkably weak compared to that of the BC22 and BCG183 /BcKMO. Expression patterns of BcKMO and MAPK signaling pathway key genes bmp1 and bmp3 were detected. The expression levels of BcKMO and bmp3 were stronger in 7 d mycelia and sclerotia of BC22. The expression levels of BcKMO, bmp1,and bmp3 were stronger in BC22 cultured on medium with sucrose or fructose. Real-time PCR technology was used to detect the expression levels of bmp1 and bmp3 in the BcKMO gene mutants,and the BcKMO expression levels in RNAi mutant of bmp1 and bmp3. The expression level of bmp1 was significantly upregulated in the BCG183 mutant,the expression level of bmp3 was significantly downregulated in the BCG183 mutant. The expression level of BcKMO in the RNAi mutants of bmp1 gene was obviously higher than that of BC22; the expression level BcKMO gene in the RNAi mutants of bmp3 was obviously lower than that of BC22. These results indicated that BcKMO negatively regulated the expression of bmp1 and positively regulated the bmp3 expression, bmp1 negatively regulated the BcKMO gene expression,and bmp3 positively regulated the BcKMO gene expression. Therefore,the results provide a foundation for clarifying the molecular mechanism of the BcKMO gene in growth,development and pathogenicity in Botrytis cinerea.

Key words: Botrytis cinerea, BcKMO, MAPK signaling pathway

CLC Number:

Q78
S432.1

SU Youke, YUAN Xuemei, WANG Min, ZANG Jinping, CAO Hongzhe, ZHANG Kang, DONG Jingao, ZHANG Jing, XING Jihong. Analysis of the Relationship Between BcKMO Gene and MAPK Signaling Pathway in Botrytis cinerea[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(5): 89-94. doi: 10.7668/hbnxb.2018.05.012.

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References

[1] Nakajima M,Akutsu K. Virulence factors of Botrytis cinerea[J]. Journal of General Plant Pathology,2014,80(1):15-23.
[2] Elad Y,Pertot I,Cotes Prado A M,et al. Plant hosts of Botrytis spp. Botrytis-the fungus,the pathogen and its management in agricultural systems[M]. Switzerland:Springer International Publishing,2016.
[3] Weiberg A,Wang M,Lin F M,et al. Fungal small RNAs suppress plant immunity by hijacking host RNA interference pathways[J]. Science,2013,342(6154):118-123.
[4] Amselem J,Cuomo C A,Van Kan J A,et al. Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea[J]. PLoS Genetics,2011,7(8):e1002230.
[5] Van Kan J A. Shaw M W,Grant-Downton R T. Botrytis species:relentless necrotrophic thugs orendophytes gone rogue?[J]. Molecular Plant Pathology,2014,15(9):957-961.
[6] Schumacher J. Signal transduction cascades regulating differentiation and virulence in Botrytis cinerea. Botrytis the fungus,the pathogen and its management in agricultural systems[M]. Switzerland:Springer International Publishing,2016.
[7] Marschall R,Tudzynski P. BcIqg1,a fungal IQGAP homolog,interacts with NADPH oxidase,MAP kinase and calcium signaling proteins and regulates virulence and development in Botrytis cinerea[J]. Molecular Microbiology,2016,101(2):281-298.
[8] Leroch M,Mueller N,Hinsenkamp I,et al. The signalling mucin Msb2 regulates surface sensing and host penetration via BMP1 MAP kinase signalling in Botrytis cinerea[J]. Molecular Plant Pathology,2015,16(8):787-798.
[9] Schumacher J,Tudzynski P. Morphogenesis and Infection in Botrytis cinerea morphogenesis and pathogenicity in Fungi[M]. Berlin Heidelberg:Springer Berlin Heidelberg,2012.
[10] Liu W,Souli M C,Perrino C,et al. The osmosensing signal transduction pathway from Botrytis cinerea regulates cell wall integrity and MAP kinase pathways control melanin biosynthesis with influence of light[J]. Fungal Genetics and Biology,2011,48(4):377-387.
[11] Heller J,Ruhnke N,Espino J J,et al. The mitogen-activated protein kinase BcSak1 of Botrytis cinerea is required for pathogenic development and has broad regulatory functions beyond stress response[J]. Molecular Plant-microbe Interactions,2012,25(6):802-816.
[12] Michielse C B,Becker M,Heller J,et al. The Botrytis cinerea Reg1 protein,a putative transcriptional regulator,is required for pathogenicity,conidiogenesis,and the production of secondary metabolites[J]. Molecular Plant-microbe Interactions,2011,24(9):1074-1085.
[13] Li X,Fernández-Ortuño D,Grabke A,et al. Resistance to fludioxonil in Botrytis cinerea isolates from blackberry and strawberry[J]. Phytopathology,2014,104(7):724-732.
[14] Schamber A,Leroch M,Diwo J,et al. The role of mitogen-activated protein (MAP) kinase signalling components and the Ste12 transcription factor in germination and pathogenicity of Botrytis cinerea[J]. Molecular Plant Pathology,2010,11(1):105-119.
[15] Rui O,Hahn M. The Slt2-type MAP kinase Bmp3 of Botrytis cinerea is required for normal saprotrophic growth,conidiation,plant surface sensing and host tissue colonization[J]. Molecular Plant Pathology,2007,8(2):173-184.
[16] 张玉净,郝志敏,郑蒙,等. 灰葡萄孢产孢缺陷菌株的遗传分析[J]. 华北农学报,2011,26(3):86-89.
[17] 李培芬,赵福鑫,董丽萍,等. 灰葡萄孢BcKMO 在病菌生长、发育和致病过程中的功能[J]. 中国农业科学,2014,47(15):2971-2979.
[18] 李培芬,赵福鑫,董丽萍,等. 灰葡萄孢犬尿氨酸单氧酶基因BcKMO 调控病菌致病力的机制分析[J]. 中国农业科学,2014,47(16):3169-3175.
[19] Doehlemann G,Berndt P,Hahn M. Different signalling pathways involving a Galpha protein,cAMP and a MAP kinase control germination of Botrytis cinerea conidia[J]. Molecular Microbiology,2006,59(3):821-835.
[20] Yang Q,Yan L,Gu Q,et al. The mitogen-activated protein kinase kinase kinase BcOs4 is required for vegetative differentiation and pathogenicity in Botrytis cinerea[J]. Applied Microbiology and Biotechnology,2012,96(2):481-492.

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