Cloning and Expression Analysis of GhTGA1 and GhTGA9.2 in Cotton

doi:10.7668/hbnxb.2018.05.011

Abstract

Abstract: GhTGA1 and GhTGA9.2 of transcription factor genes were isolated by RT-PCR technology from Zhongmiansuo 12 cultivar of resistance to Fusarium oxysporum. Sequence analysis showed that the ORF of GhTGA1 and GhTGA9.2 were 1 281 bp and 1 461 bp,respectively.They encoded 405 and 486 amino acids,respectively. GhTGA1 and GhTGA9.2 all contained two conserved bZIP and DOG1 domains. They belonged to the TGA transcription factor gene of bZIP subfamily. The GhTGA1 and GhTGA9.2 expression feature were detected by Real-time quantitative PCR(qRT-PCR),the results showed that GhTGA1 gene was up-regulation expressed with the duration of the treatment of Fov,ethylene (ET) and jasmonic acid (JA). In each treatment time point,the expression of GhTGA1 gene was lower than that of Mock with the treatment of salicylic acid (SA). We speculated that GhTGA1 gene might be involved in ethylene (ET) and jasmonic acid (JA) signaling pathway and regulated resistance to Fusarium oxysporum in cotton; After the treatment of Fusarium oxysporum,the expression of GhTGA9.2 gene was lower than Mock at different treatment time points,and was down-regulated expression. In other hand,it was up-regulation expressed with the duration of the treatment of hormone (ET,JA,SA).It suggested that GhTGA9.2 might participate in stress response through hormone (ET,JA and SA) pathways. The analysis of gene expression characteristics of GhTGA1 and GhTGA9.2 provides a basis for further study.

Key words: Cotton, TGA transcription factor, Gene clone of GhTGA1 and GhTGA9.2, Expression analysis

CLC Number:

Q78
S562.03

ZHAO Zengqiang, LI Xiaoling, ZHANG Xi, ZHANG Wei, LIAN Wenming. Cloning and Expression Analysis of GhTGA1 and GhTGA9.2 in Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(5): 82-88. doi: 10.7668/hbnxb.2018.05.011.

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