Cloning and Analyzing the Function of the Promoter of an Effector Homologous of Chorismate Mutase in Sclerotinia sclerotiorum

doi:10.7668/hbnxb.2018.05.013

Abstract

Abstract: In order to investigate the mechanism of the effector homologous of chorismate mutase in Sclerotinia sclerotiorum,the promoter was cloned and analyzed. The promoter element and the Cis element were analyzed through the online software,Promoter 2.0 and Promoter scan,then the DNA sequence of the promoter was cloned by the method of PCR and the GFP fusion vector were constructed,transformed in the protoplast of Sclerotinia sclerotiorum. In the end,the GFP fluorescence were detected through confocal microscope. As a result,TATA box and CAAT box were found in the upstream of the gene,and a 733 bp DNA sequence upstream of ATG was cloned by PCR using the primer XS1-1 and XS1-2,then the GFP fusion vector were constructed based on the plasmid of pBluntNAT-GFP,through transforming the protoplast of Sclerotinia sclerotiorum,some transformants were obtained in which the GFP fluorescence were detected through confocal microscope. All these indicated that the 733 bp DNA sequence upstream of ATG could act as the promoter of the gene.

Key words: Sclerotinia sclerotiorum, Chorismate mutase, Effector, Promoter

CLC Number:

CHANG Xue, SHENG Yinsheng, REN Aizhi, ZHAO Peibao. Cloning and Analyzing the Function of the Promoter of an Effector Homologous of Chorismate Mutase in Sclerotinia sclerotiorum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(5): 95-98. doi: 10.7668/hbnxb.2018.05.013.

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