TAIL-PCR System for Isolation of an Unlmo wn DNA Sequence Lying Inside the 3' Noncoding Region of the Fibroin Gene

doi:10.7668/hbnxb.2009.01.024

Abstract

Abstract: 1998 bP DNA sequence was isolated here with genomic DNA of Antheraea pernyd by Them}al Asymmetric Interlaced PCR(TAIL-PCR)The results from comparing 1998 bp DNAsequence with the sequence that alrea landed onthe Genebank(AF083334)showed that the DNA sequence(1 758 bp)in the newly isolated DNA segunts DNA sequence lying inside the 3 noncoding region of the fibroin gene. The method we describe here provides a simple,was an unknownrapid and efficient means to isolate an unknown DNA sequence lying inside the 3' noncoding region of the fibroingene.

Key words: TAIL-PCR, Fibroin gene, Degenerate primer, Flanking sequences

CLC Number:

LENG Chun-ling. TAIL-PCR System for Isolation of an Unlmo wn DNA Sequence Lying Inside the 3' Noncoding Region of the Fibroin Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 108-111. doi: 10.7668/hbnxb.2009.01.024.

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