Change in Glycerol-3-Phosphate Acyltransferase Activity of Lilium dauricum Ker Grawl.and Cloning of Its Conserved cDNA Region under Cold Stress

doi:10.7668/hbnxb.2011.05.008

Abstract

Abstract: Change in Glycerol-3-Phosphate Acyltransferase(GPAT)activity of Lilium dauricum under cold stress was explored and the conserved sequence of GPAT was amplified using degenerate primers.The results demonstrated that the conserved sequence was 744 bp in length,which was deduced coding 247 amino acids.There is a highly conserved domain(WIAPSGGRDRP)in the amino acid sequence of GPAT.Aligning the conserved domain with BLAST of NCBI,we found it was a catalytic domain of LPLAT gene superfamily enzymes.This family mostly catalyzed acyl-coenzyme A(acylCoAs)or acyltransferase which was acyl in the Acyl carrier protein(acylACPs)binding with receptor protein.The gene accession nucleotide sequence number in GenBank was bankit1413285.

Key words: Lilium dauricum, Cold stress, Glycerol-3-Phosphate acyltransferase, Degenerate primers, Conserved cDNA region, Homology

CLC Number:

S682.29

CHEN Li-jing, GE Ling, LI Hao-ge, GUO Zhi-fu, ZHANG Li, DANG Xiao-xuan, TAO Cheng-guang, LI Tian-lai. Change in Glycerol-3-Phosphate Acyltransferase Activity of Lilium dauricum Ker Grawl.and Cloning of Its Conserved cDNA Region under Cold Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(5): 34-39. doi: 10.7668/hbnxb.2011.05.008.

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