Triplex-RT-PCR Detection of PVY,PVS and PLRV in Potato

doi:10.7668/hbnxb.2011.05.009

Abstract

Abstract: Three pairs of primers for PVY,PVS and PLRV,by comparing and finding out each conserved domain of CP sequence were designed,and then triplex-RT-PCR technology was used to amplify three product fragments of PVY,PVS and PLRV in one reaction simultaneously.The length of the PVY,PVS and PLRV are fit to the length of target DNA,781,181,364 bp respectively.The homology of the three viruses all reach up to 95% by comparing sequencing result with each published sequences in GeneBank respectively.After diluting the RNA samples,the minimum detection content for PVY,PVS and PLRV is 4.5 pg/μL-4.5 fg/μL,3.7 fg/μL and 4.6 fg/μL in RT-PCR respectively.Triplex-RT-PCR technology provides one convenient and high-efficiency molecular method to detect potato materials infected with one or more viruses among PVY,PVS and PLRV.

Key words: Potato, Virus detection, Triplex-RT-PCR

CLC Number:

S435.32

ZHANG Hua-peng, ZHANG Jian-feng, LIU Jun-ying, WANG Cong-cong. Triplex-RT-PCR Detection of PVY,PVS and PLRV in Potato[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(5): 40-45. doi: 10.7668/hbnxb.2011.05.009.

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