Cloning and Sequence Analysis of a DNA Encoding Ethylene ReportorErs1 from Starwberry

doi:10.7668/hbnxb.2011.05.010

Abstract

Abstract: In this study a ripening related ethvlene recepor Ers1 gene in strawberry fruits was cloned for the following-up reseach on gene and improving varieties of strawberry through transgenic technology.A pair of primers were designed and synthesized based on the sequence reported of Chandler-Ers1.Using genome DNA extracted from All-star strawberry mature fruits as template the specific PCR product of Ers1 was obtained,then it was ligated to pEGM-T easy vector and sequenced.The sequencing data showed that the PCR product was 667 bp encoding 222 peedicted amino acid residues.Comparison with the cDNA sequence from Chandler-Ers1 indicated the homlogy was 98%,and amino acid identitise were 97%.

Key words: Strawberry, Ethylene receptor Ers1, Gene clone, Sequence analysis

CLC Number:

S668.4

LIU Si-si, MA Jun-lian, TANG Xia, ZHANG Zi-de, SONG Chun-li. Cloning and Sequence Analysis of a DNA Encoding Ethylene ReportorErs1 from Starwberry[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(5): 46-49. doi: 10.7668/hbnxb.2011.05.010.

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http://www.hbnxb.net/EN/Y2011/V26/I5/46

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