Study on Transformation of Starch Branching Enzyme SBEIIb Gene into Maize Inbred Line

doi:10.7668/hbnxb.2011.05.011

Abstract

Abstract: The SBEIIb gene was transferred into maize inbred line Danhuang 25 by Agrobacterium tumefaciens mediated transformation.Factors influenced transformation efficiency was studied.The results showed that 9 days with subculture of callus,AS concentration in the bacilli concentration at 10 mg/L,20 minutes infection time and co-culture 60 hours were the optimal conditions.PCR analysis on four resistance plants demonstrated that three of them were positive and the foreign gene was integrated into maize genome.

Key words: Maize, Agrobacterium tumefaciens mediated, Conversion conditions, SBEIIb gene

CLC Number:

S513

CHE Xin-ming, GUO Xin-mei, PEI Yu-he, GAO Xue-yan, SONG Xi-yun. Study on Transformation of Starch Branching Enzyme SBEIIb Gene into Maize Inbred Line[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(5): 50-53. doi: 10.7668/hbnxb.2011.05.011.

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http://www.hbnxb.net/EN/Y2011/V26/I5/50

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