Cloning and Expression Analysis of Two Late Embryogenesis Abundant(LEA) Proteins from Upland Cotton

doi:10.7668/hbnxb.20190096

Abstract

Abstract: The LEA genes often exist as a large gene family in higher plants, and have the pivotal role in plant growth and development, and various stress response. To further study the function of cotton LEA genes, two LEA genes were isolated from a cotton fiber-normolized cDNA library, which were named GhLEA and GhLEAG1 (GenBank accession number KF906314 and KF906316). GhLEA contained an open reading frame(ORF) of 948 bp, encoding a protein of 315 amino acids, with a molecular weight of 35 ku, and a theoretical isoelectric point of 4.4. GhLEAG1 contained an open reading frame(ORF) of 756 bp, encoding a peptide of 251 amino acids, with a molecular weight of 27 ku, and a theoretical isoelectric point of 10.76. Phylogenetic tree showed that GhLEA and GhLEAG1 had different motif elements, belonging to LEA2 and LEA3, separately. Quantitative RT-PCR analysis showed that both GhLEA and GhLEAG1 had the expression peak at 20DPA, and the expression of GhLEA in root was the highest, but the expression of GhLEAG1 was the highest in stem. The expression patterns of the two genes were significantly different under salt stress and PEG. In addition, the two genes were also induced by ABA, the expression level was increased first and then decreased. The data indicated that GhLEA and GhLEAG1 might play an important role in the abiotic stress response of cotton. This study will provide an important reference for further research on the molecular function of LEA in cotton.

Key words: Cotton, LEA, Abiotic stress, Sequence analysis, Expression analysis

CLC Number:

Q78
S562.03

ZHEN Junbo, LIU Di, SONG Shijia, LIU Linlin, CAI Xiao, ZHANG Xi, LI Haishan, CHI Ji. Cloning and Expression Analysis of Two Late Embryogenesis Abundant(LEA) Proteins from Upland Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 33-38. doi: 10.7668/hbnxb.20190096.

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