Acta Agriculturae Boreali-Sinica ›› 2025, Vol. 40 ›› Issue (3): 133-140. doi: 10.7668/hbnxb.20195360

Special Issue: Cotton Plant protection Biotechnology

• Resources & Environment·Plant Protection • Previous Articles     Next Articles

Functional Analysis of Fusarium wilt Resistance Gene GhERF14 in Cotton

ZHAO Longfei1,2, ZHANG Jianhua3, ZHAO Zengqiang4, CAI Shudong2, ZHANG Wei1,   

  1. 1 Agriculture College,Shihezi University,Shihezi 832000,China
    2 Hami Animal Husbandry Station,Hami 839000,China
    3 College of Horticultural Science & Technology,Hebei Normal University of Science & Technology,Changli 066600,China
    4 Cotton Research Institute of Xinjiang Academy of Agricultural and Reclamation Sciences,Shihezi 832000,China
  • Received:2024-11-19 Published:2025-06-28

Abstract:

This study explored the relationship between cotton GhERF14 gene and Fusarium oxysporum pathogenicity,analyzed the molecular mechanism of F.oxysporum pathogenicity,and tentatively explored the response of cotton GhERF14 gene to Fusarium wilt disease and its regulatory effect on related resistance genes,to provides some theoretical basis for breeding new cotton cultivars resistant to wilt.Gene cloning and virus-induced gene silencing(VIGS)were used to construct the non-conserved domain interference vector pTRV2-GhERF14.Using Real-time fluorescence quantification(qRT-PCR)technology and VIGS technology,the expression characteristics of GhERF14 and downstream genes related to lignin,ethylene(ET),jasmonic acid(JA),salicylic acid(SA),antioxidant enzymes and disease progression-related protein(PR)were analyzed after F.oxysporum stress and hormone treatment,and the role of GhERF14 in the process of cotton disease resistance was analyzed.The results indicated that inhibition of GhERF14 gene expression could significantly reduce the synthesis of jasmonic acid(JA),salicylic acid(SA)and ethylene(ET)and the expression of genes related to the signaling pathway.After GhERF14 gene silencing by VIGS technology,cotton plants were more susceptible to Fusarium wilt.These results suggested that GhERF14 may play an important role in the pathogenesis and host-pathogen interaction of F.oxysporum.

Key words: Cotton, GhERF14, Blight, VIGS, Fusarium oxysporum

CLC Number: 

Cite this article

ZHAO Longfei, ZHANG Jianhua, ZHAO Zengqiang, CAI Shudong, ZHANG Wei. Functional Analysis of Fusarium wilt Resistance Gene GhERF14 in Cotton[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3): 133-140. doi: 10.7668/hbnxb.20195360.

share this article