Cloning and Expression Analysis of Myb Transcription Factor NtBL1 Gene in Nicotiana tabacum

doi:10.7668/hbnxb.20190004

Abstract

Abstract: In order to discover the function of Blind gene in Nicotiana tabacum, we cloned one Blind-like gene, NtBL1, from K326 by homologue cloning method. The protein domain and structure was predicted by PROSITE(ExPASy), Sopma and Swissmodel, the Phylogeny tree construction and Motif prediction of NtBL1 and its homologues were analyzed separately by Mega 7.0 and MEME, the expression pattern of NtBL1 in different tissues was analyzed by semi-quantitative PCR, and the expression changes of NtBL1 under abiotic stress treatment was detected by qRT-PCR. The results showed that the coding sequence of NtBL1 was 1 014 bp, encoding 337 aa. The expression pattern of NtBL1 in transcription level was generally detected in several organs and growing stages, while it had the highest expression level in root. Bioinformation analysis showed that NtBL1 was a typical R2R3 Myb protein, which contained 2 Myb-type HTH domains. The phylogenetic tree analysis showed that NtBL1 gene was shared lots of motifs with tomato Blind. The results of qRT-PCR showed that the expression level of NtBL1 gene was up-regulated by ABA treatment and the highest expression level appeared in 24 h treatment; The expression level of NtBL1 gene was down-regulated by NaCl treatment and the lowest expression level presented in 24 h treatment. In conclusion, NtBL1 gene might participate in regulation of axillary mersitem development by ABA and NaCl.

Key words: Tobacco, NtBL1, Myb gene family, Gene expression

CLC Number:

Q78
S572.03

LIU Yajie, XUE Chong, QIU Shirui, LI Xiang, TANG Liwen, ZENG Shuhua. Cloning and Expression Analysis of Myb Transcription Factor NtBL1 Gene in Nicotiana tabacum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 26-32. doi: 10.7668/hbnxb.20190004.

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References

[1] Chomicki G, Coiro M, Renner S S. Evolution and ecology of plant architecture:integrating insights from the fossil record, extant morphology, developmental genetics and phylogenies[J]. Annals of Botany, 2017, 120(6):855-891.doi:10.1093/aob/mcx113.
[2] Wai A H, An G. Axillary meristem initiation and bud growth in rice[J]. Journal of Plant Biology, 2017, 60(5):440-451. doi:10.1007/s12374-017-0088-x.
[3] Janssen B J, Drummond R S M, Snowden K C. Regulation of axillary shoot development[J]. Curr Opin Plant Biol, 2014, 17:28-35. doi:10.1016/j.pbi.2013.11.004.
[4] Žádníková P, Simon R. How boundaries control plant development[J]. Current Opinion Plant Biology, 2014, 17:116-125. doi:10.1016/j.pbi.2013.11.013.
[5] Long J, Barton M K. Initiation of axillary and floral meristems in Arabidopsis[J]. Development Biology, 2000, 218(2):341-353. doi:10.1006/dbio.1999.9572.
[6] Schumacher K, Schmitt T, Rossberg M, Schmitz C, Theres K. The Lateral suppressor(Ls) gene of tomato encodes a new member of the VHIID protein family[J]. Proc Natl Acad Sci, 1999, 96(1):290-295. doi:10.1073/pnas.96.1.290.
[7] Greb T, Clarenz O, Schāfer E, Müller D, Herrero R, Schmitz G, Theres K.Molecular analysis of the LATERAL SUPPRESSOR gene in Arabidopsis reveals a conserved control mechanism for axillary meristem formation[J]. Gene Dev, 2003, 17:1175-1187. doi:10.1101/gad.260703.
[8] Zhang B, Liu X, Xu W N, Chang J Z, Li A, Mao X G, Zhang X Y, Jing R L. Novel function of a putative MOC1 ortholog associated with spikelet number per spike in common wheat[J]. Scientific Reports, 2015, 5:12211. doi:10.1038/srep12211.
[9] Komatsu M, Maekawa M, Shimamoto K, Kyozuka J. The LAX1 and FRIZZY PANICLE2 genes determine the inflorescence architecture of rice by controlling rachis-branch and spikelet development[J]. Dev Biol, 2001, 231(2):364-373. doi:10.1006/dbio.2000.9988.
[10] Ritter M K, Padilla C M, Schmidt R J. The maize mutant barren stalk1 is defective in axillary meristem development[J]. American Journal of Botany, 2002, 89(2):203-210. doi:10.3732/ajb.89.2.203.
[11] Komatsu K, Maekawa M, Ujiie S, Satake Y, Furutani I, Okamoto H, Shimamoto K, Kyozuka J. LAX and SPA:major regulators of shoot branching in rice[J]. Proc Natl Acad Sci, 2003, 100(20):11765-11770. doi:10.1073/pnas.1932414100.
[12] Gallavotti A, Zhao Q, Kyozuka J, Meeley R B, Ritter M K, Doebley J F, Pè E M, Schmit R J. The role of barren stalk1 in the architecture of maize[J]. Nature, 2004, 432(7017):630-635. doi:10.1038/nature03148.
[13] Yang F, Wang Q, Schmitz G, Müller D, Theres K. The bHLH protein ROX acts in concert with RAX1 and LAS to modulate axillary meristem formation in Arabidopsis[J]. Plant J, 2012, 71(1):61-70. doi:10.1111/j.1365-313X.2012.04970.x.
[14] Keller T, Abbott J,Moritz T, Doerner P. Arabidopsis REGULATOR OF AXILLARY MERISTEMS1 controls a leaf axilstem cell niche and modulates vegetative development[J]. The Plant Cell, 2006, 18:598-611. doi:10.1105/tpc.105.038588.
[15] Schmitz G, Tillmann E, Carriero F, Fiore C, Cellini F, Theres K. The tomato blind gene encodes a MYB transcription factor that controls the formation of lateral meristems[J]. Proc Natl Acad Sci, 2002, 99(2):1064-1069. doi:10.1073/pnas.022516199.
[16] Müller D, Schmitz G, Theres K. Blind homologous R2R3 Myb genes control the pattern of lateral meristem initiation in Arabidopsis[J]. The Plant Cell, 2006, 18(3):586-597. doi:10.1105/tpc.105.038745.
[17] Busch B L, Schmitz G, Rossmann S, Piron F, Ding J, Bendahmane A, Theres K. Shoot branching and leaf dissection in tomato are regulated by homologous gene modules[J]. The Plant Cell, 2011, 23(10):3595-3609. doi:10.1105/tpc.111.087981.
[18] 李文正,高玉龙,宋中邦,李永平,王丙武.烟草NtMAX4-1基因的克隆及特征分析[J].基因组学与应用生物学, 2016, 35(11):3147-3153. doi:10.13417/j.gab.035.003147.
Li W Z, Gao Y L, Song Z B, Li Y P, Wang B W. Cloning and characteristic analysis of NtMAX4-1 gene in tobacco[J]. Genomics and Applied Biology, 2016, 35(11):3147-3153.
[19] 陈雅琼,孙亭亭,陈蕾,丁安明,陈涣,孙玉合.烟草BRANCHED1-Like基因的克隆及表达分析[J].植物遗传资源学报, 2015, 16(6):1321-1329. doi:10.13430/j.cnki.jpgr.2015.06.026.
Chen Y Q, Sun T T, Chen L, Ding A M, Chen H, Sun Y H. Molecular cloning and expression analysis of BRANCHED1-Like gene in common tobacco[J]. Journal of Plant Genetic Resources, 2015, 16(6):1321-1329.
[20] 陈雅琼.烟草腋芽发育相关基因的克隆与功能分析[D].北京:中国农业科学院, 2015.doi:10.7666/d.Y2787367.
Chen Y Q. Cloning and functional analysis of axillary bud development related genes in tobacco[D]. Beijing:Chinese Academy of Agricultral Sciences, 2015.
[21] 陈蕾.烟草NtMAX3和NtMAX4基因克隆及RNA干扰研究[D].北京:中国农业科学院,2016.
Chen L. Cloning and RNA interference of NtMAX3 and NtMAX4 gene in Nicotiana tabacum[D]. Beijing:Chinese Academy of Agricultral Sciences, 2016.
[22] 王姗姗,杨军,王中,林福呈,潘婷,武明珠,张剑锋,曹培健,谢小东.利用CRISPR/Cas9技术的烟草NtLS基因敲除分析[J].烟草科技, 2018, 51(2):1-8. doi:10.16135/j.issn1002-0861.2017.0363.
Wang S S, Yang J, Wang Z, Lin F C, Pan T, Wu M Z, Zhang J F, Cao P J, Xie X D. Targeted mutagenesis of NtLS gene by using CRISPR/Cas9 system[J]. Tobacco Science&Technology, 2018, 51(2):1-8.
[23] Yu Y T, Wu Z, Lu K, Bi C, Liang S, Wang X F, Zhang D P. Overexpression of the MYB37 transcription factor enhances abscisic acid sensitivity, and improves both drought tolerance and seed productivity in Arabidopsis thaliana[J]. Plant Mol Biol, 2016, 90(3):267-279. doi:10.1007/s11103-015-0411-1.

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