Cloning and Bioinformatics Analysis of LPAT4 in Rapeseed

doi:10.7668/hbnxb.2014.03.011

Abstract

Abstract: To further study on LPAT4 of Brassica napus, a pair of primers were designed to obtain the full length CDs sequence of rapeseed LPAT4 gene, according to the homologous comparison of B.campestris, B.oleracea and assembled EST sequence, which was strong similarity to the LPAT4 gene of Arabidopsis thaliana by searching bioinformatics at Geboc database.In this paper, two copies of full length CDs sequences(1 143, 1 140 bp)have been cloned, these clones were designated as BnLPAT4-1 and BnLPAT4-2, respectively. BnLPAT4-1 encoded a protein of 380 amino acids with the molecular weights of 43.29 kDa and the isoelectric point of 8.74; BnLPAT4-2 encoded a polypeptide of 379 amino acids with the molecular weights of 43.16 kDa and the isoelectric point of 8.93.They belong to the LPLAT superfamily with LPLAT_LCLAT1 like domain.SNP analysis between different rapeseed cultivars revealed that LPAT4 exist 41 polymorphic sites, In which 7 were singleton polymorphic sites, and 34 were parsimony informative polymorphic sites.41 polymorphic sites leaded to 41 mutations, among them, 23 were synonymous changes and 18 were replacement changes which contribute to 16 amino acids changed.In terms of sequence composition, physico-chemical property, conserved domain and homologous-modeling analysis, the analyses showed that BnLPAT4-1 and BnLPAT4-2 were two copies of Xiangyou 15 LPAT4 gene, at the same time, they should be the members of LPLAT superfamily.

Key words: LPAT4, Brassica napus L., Sequence analysis, Homology-modeling analysis, SNP

CLC Number:

Q78
S635.03

XIAO Dan-wang, LIU Cong, HU Xue-fang, WU Ke-bin, GUAN Chun-yun, XIONG Xing-hua. Cloning and Bioinformatics Analysis of LPAT4 in Rapeseed[J]. journal1, 2014, 29(3): 52-58. doi: 10.7668/hbnxb.2014.03.011.

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