Cloning and Bioinformatics Analysis of Porcine Sapovirus CH430 Non-structural Protein p70 Gene

doi:10.7668/hbnxb.2014.03.010

Abstract

Abstract: The non-structural protein p70 gene of PoSaV CH430 strain was cloned by RT-PCR and then sequenced in our study.The results showed that the full length of p70 gene was 1 995 nt encoding a protein of 665 aa.Homology alignment and evolutionary tree showed that PoSaV CH430 strain belong to GⅢ.The bioinformatics analysis demonstrated that the isoelectric point and molecular weight of non-structural protein p70 were 5.96 and 72 876.6 u.The protein had no signal peptide and transmenbrane domain.There were 25 phosphorylation sites including 6 Sers, 13 Thrs and 6 Tyrs.Protein phosphorylation was concerned with signal transduction, so this protein may be a signaling molecule.There were Trypsin-like serine proteases and RdRp functional domain.Main structure of trypsin-like serine proteases functional domain was a column structure and an incomplete β-barrel.Main structure of RdRp functional domain was a fold whose organisation has been likened to the shape of a right hand with three subdomains termed fingers, palm and thumb.

Key words: Porcine sapovirus, Non-structural protein p70, Clone, Bioinformatics analysis

CLC Number:

Q78
S432

LIU Wei, WANG En-li, LIU Ji-xing, YANG Bin, LAN Xi. Cloning and Bioinformatics Analysis of Porcine Sapovirus CH430 Non-structural Protein p70 Gene[J]. journal1, 2014, 29(3): 46-51. doi: 10.7668/hbnxb.2014.03.010.

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