Chilling Tolerance Analysis of Ethylene Responsive Mutants in Tobacco

doi:10.7668/hbnxb.2016.06.018

Abstract

Abstract: To study the regulatory function of ethylene in plant stress tolerance,over 40 ethylene insensitive tobacco mutants were obtained from a genetic screen based on the typical seedling phenotype to ethylene in darkness,named "triple response",and most of the mutants displayed chilling resistance.The present study chose 4 mutant strains for further analyses in their chilling tolerance,physiological basis,and cold-regulated gene expressions.Under low temperature (0℃) stress,the electrolyte permeability of the tobacco ethylene insensitive mutants was significantly lower than that of the wild type,while the activities of SOD,POD and catalase were higher than those of the wild type.Further study indicated that the cold-induced genes showed highly transcription level under low temperature (0℃) stress. And,the transcripts levels of the downstream ERFs in the ethylene signal transduction pathway in the mutants were altered in the tobacco mutants.These data provide a direct molecular evidence for the strong resistance of the tobacco ethylene insensitive mutants to chilling stress.This study not only provides a new evidence for the relationship between ethylene signaling and chilling stress,but also lays a foundation for the use of biotechnology to improve crop stress resistance.

Key words: Tobacco, Cold stress, Ethylene, Chilling tolerance, Mutant

CLC Number:

S572.01

ZHAO Yuhang, LI Fengming, ZHANG Tao, HOU Xiaomin, YANG Hongbing, DONG Chunhai. Chilling Tolerance Analysis of Ethylene Responsive Mutants in Tobacco[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(6): 111-118. doi: 10.7668/hbnxb.2016.06.018.

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