Construction and Analysis of VP3 Protein from A Foot-and-mouth Disease Virus Strain OA/58

doi:10.7668/hbnxb.2008.06.006

Abstract

Abstract: Foot2and2mouth disease virus strain OA/ 58 RNAs were used as templates for RT2PCR. The amplified cD2 NA products were cloned into pMD182T vectors and transformed into JM109. The recombinant plasmids were ident ified by electrophoresis, PCR, and analysis of tow cleavages with BamH I and Hind III. After analyzing the difference among VP1, VP2, VP3, VP4, at the nucleotide level, the mutation rates of these 4 encoding sequences were no difference (p> 0. 05), however, at the amino acid level, those mutation rates were different (p< 0. 05). The regions of 1- 24th, 26- 35th, 37- 43th, 45- 57th, 61- 122th, 124- 173th, 175- 209th and 211- 220th in VP3 protein most probably are conservat ive. Depending on homology modeling the FMDV strain OA/ 58 VP3 protein, the 3D mold was gained. And this 3D mold was divided into A, B and C regions. This research should be used as an instruction in order to direct the work on FMDV VP3 protein.

Key words: Foot-and-mouth disease virus, VP3 protein, Homology modeling

CLC Number:

Q945

ZHOU Jian-hua, CONG Guo-zheng, GAO Shan-dian, CHANG Hui-yun. Construction and Analysis of VP3 Protein from A Foot-and-mouth Disease Virus Strain OA/58[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 28-31. doi: 10.7668/hbnxb.2008.06.006.

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