Abstract: We construct containing the expected mutations in foot-and-mouth disease virus( FMDV) vaccine strain O/HN/93 full-length cDNA clone by using site-directed mutagenesis.The recombinant plasmids containing O/HN/93 full-length genome were linearized with Not I enzyme and cotransfected into BHK-21 cells with pcDNAT7P plasmids that could express T7 RNA polymerase to rescue virus.The transfected cells were serily passaged, and the third passage showed apparent cytopathogenicity effect( CPE) within 50 h, the CPE were observed after 10 h in the fourth passage.The results of the RT-PCR, indirect immunofluorescence, electron microscope confirmed the success of the rescue out of FMDV O/HN/93 strain.The recovered virus contained genetic tags.Successfully obtained infectious full-length cDNA clone of O/HN/93 strain, It lay a foundation for further study of the candidate vaccine strain which covers a wider spectrum of antigens over pandemic strain．

Key words: Foot-and-mouth disease virus( FMDV), Vaccine strain, Infectious cDNA clones, Virus rescue

CLC Number: 

• Q78