Cloning and Expression of PGIP Gene from Cucumis melon L.

doi:10.7668/hbnxb.2010.03.009

Abstract

Abstract: A target fragment with a full length of 978 bp was amplified with genomic DNA of Cucumis melon L leaves as the templates and the conservative sequences of PGIP gene as the primers.This sequence had a full open reading frame encoding the polygalacturonase-inhibiting protein.The total ORF were comprised by 975 bp of deoxynucleotide encoding 325 amino acid.A conserved leucine-rich fragmenthad existed in the derived protein sequence.Sequencing analysis showed that it was 100% identical with the sequences of PGIP gene which had been cloned.RTPCR analysis showed that the PGIP gene was expressed in roots, stems, leaves and fruits of Cucumis melon L.As a result, a gene resource was provided for molecular breeding of plants．

Key words: Cucumis melon L．, PGIP Gene, Genetic cloning, Expression

CLC Number:

Q786

DUAN Yu-juan, GUO Qing-xun, LIU Zhi-wei, SONG Yang, HUAI Feng-tao. Cloning and Expression of PGIP Gene from Cucumis melon L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(3): 38-42. doi: 10.7668/hbnxb.2010.03.009.

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