Abstract: Based on the gE and gH genes of pseudorabies virus (PRV), two pairs of special primers were de- signed, respectively, single PCR assays for gE and gH genes were established. Under the optimized amplification conditions, the duplex PCR method was established to differentiate virulent and vaccine strains of pseudorabies vi- rus. In a duplex PCR assay, two fragments of 429 bp for gE gene and 355 bp for gH gene were simultaneously am- plified from pseudorabies virus virulent strains DNA, only a 355 bp fragment for gH gene was amplified from pseud- orabies virus vaccine strains DNA, whereas no PCR products were amplified from porcine circovirus type 2 and por- cine parvovirus. Then the detection limit of duplex PCR was estimated to be 1 × 10²TCID₅₀ /0. 1 mL. This method is suitable for differentiation of wild-type pseudorabies virus and vaccine strains in clinical specimen.

Key words: Pseudorabies virus, Duplex PCR, Detection, Virulent strains, Vaccine strains

CLC Number: 

• S828