Abstract: In order to detect and identify the viral causing agent that infects tomato vegetable plants in China, a total of 431 susceptible tomato plant samples were collected from different vegetable planting areas during 2015-2018. Total RNA were subjected to detect the viruses using reported specific primers of 12 main viruses and demonstrated by reverse transcription-PCR(RT-PCR). The TYLCV had the highest detection rate(65.20%). ToCV, TMV, CMV, ToMV, TSWV and PVY were popular, having the detection rates of 21.11%, 20.19%, 17.63%, 15.31%, 14.15% and 11.37%, respectively. No TICV, PVX, TYLCVNB and BBWV was detected. The complex infection phenomenon showed that the compound infection rate with two viruses accounted for 52.60% of the total, the combined infection rate with three viruses accounted for 27.92%, and the combined infection rates with four viruses and five viruses was slightly lower, accounting for 0.65% and 18.83%, respectively. Based on the detection data of tomato viruses from 20 regions of China, the most detected viruses were in Beijing, of which 7 viruses were detected. Five viruses were detected in Shandong Province. Four viruses were detected in Henan, Gansu and Ningxia. Three viruses were detected in Xizang, Hebei, Zhejiang, Shaanxi and Hainan. Two viruses were detected in Sichuan, Tianjin, Heilongjiang, Shanxi and Neimenggu, while only one virus was detected in other areas. Phylogenetic trees were constructed with partial gene sequences of TYLCV and TSWV, respectively. The isolates of TYLCV from Beijing(MK336782)and Hunan had the closest relationship, and the isolates of TSWV from Henan(MK318839)and Shandong shared the closest relationship.

Key words: Tomato, Virus diseases, Tomato yellow leaf curl virus (TYLCV), Tomato chlorosis virus (ToCV), Complex infection, Molecular detection

CLC Number: 

• S432.4