Development of a Triple PCR Method for Identifying Three Genotypes of Porcine bocavirus

doi:10.7668/hbnxb.2016.02.003

Abstract

Abstract: To develop a method for identifying three genotypes of Porcine bocavirus, 3 pairs of primers against conserved regions were designed according to the published gene sequences of Porcine bocavirus, and the PCR products were 645 bp(PBoV1), 352 bp(PBoV2), 259 bp(PBoV3), respectively.By optimizing the reaction conditions, a triple PCR method for detecting all three genotypes of Porcine bocavirus was established for the first time.Results showed that no specific band was amplified from other porcine viruses by the triple PCR.The detection limit of the method was 8×10^-7 ng/μL.The results indicated that this method had not only good sensitivity but also high specificity.The clinical samples were used to detect three genotypes of Porcine bocavirus by the triple PCR and single PCR respectively with the coincidence of 100%.The establishment of the triple PCR method is helpful for identifying the three genotypes of Porcine bocavirus.

Key words: Porcine bocavirus, Genotype, Triple PCR, Detection

CLC Number:

Q78
S436

HONG Chunhui, YANG Bing, TAN Xiangjie, ZHOU Hongzhuan, XU Fuzhou, SU Xia, XUE Jing, ZHANG Xiaodong, WANG Jinluo. Development of a Triple PCR Method for Identifying Three Genotypes of Porcine bocavirus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 12-16. doi: 10.7668/hbnxb.2016.02.003.

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