Cloning of Mating Type Genes from Foxtail Millet Blast and Molecular Detection of Magnaporthe oryzae Mating Type Genes in Different Region

doi:10.7668/hbnxb.2018.03.010

Abstract

Abstract: To explore the mating type genes and distribution of foxtail millet blast in different millet production areas in China is important for the analysis of population structure of Magnaporthe oryzae. The three pairs of primers were designed according to the partial sequences of MAT1-1-1 (GenBank accession No.AB080672.2)and MAT1-2-1 (GenBank accession No.AB080673.2) genes of M. oryzae infecting rice,and the mating type genes of 186 strains of M. oryzae of foxtail millet was detected by PCR technique. In addition,amino acid sequence of α-box domain and highly mobility group protein-box of mating type genes were compared between M. oryzae of rice and M. oryzae of foxtail millet. The results showed that the amplification of mating type gene MAT1-1-1 was better by primer JPX1-1-S3/JPX1-1-A3,while the amplification of mating genotype MAT1-2-1 by JPX1-2-S1/JPX1-2-A1 was better. Amino acid sequence of α-box domain and highly mobility group protein-box of two mating type genes between M. oryzae of rice and M. oryzae of foxtail millet were highly similarity,but no identical. Mating type genes of 186 single ascospore isolates of M. oryzae in millet collected from Hebei,Shandong,Shanxi,Shaanxi,Jilin,Heilongjiang,Liaoning,Inner Mongolia,Xinjiang and Hainan Province in 2010-2016 were detected with primer JPX1-1-S3/JPX1-1-A3 and JPX1-2-S1/JPX1-2-A1. In the summer valley,the mating type of MAT1-1 was 69.15%,while the mating type MAT1-2 only accounted for 26.60%,the other 4.25% strains were double mating type strains. In the spring valley,the mating type of MAT1-1 was 38.55%,while the mating type MAT1-2 only accounted for 56.63%,the other 4.82% strains were double mating type strains. Conclusion,established the mating type gene of M. oryzae PCR detection system. Most of the millet growing areas were detected by this method have MAT1-1 and MAT1-2 two mating types,overall,the ratio of two mating type strains was close to 1:1. However,there were some differences in the mating type strains in different areas,and there were two strains of both mating type genes in nature. Rapid detection of mating type alleles and distribution of mating type genes in different regions of M. oryzae from foxtail millet is important for the study of population structure and genetic analysis of M. oryzae.

Key words: Magnaporthe oryzae, Mating type, PCR detection

CLC Number:

Q93
S432.1

REN Shilong, YANG Zhenli, BAI Hui, WANG Yongfang, QUAN Jianzhang, DONG Zhiping, LI Zhiyong, XING Jihong. Cloning of Mating Type Genes from Foxtail Millet Blast and Molecular Detection of Magnaporthe oryzae Mating Type Genes in Different Region[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(3): 56-62. doi: 10.7668/hbnxb.2018.03.010.

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